Human Embryonic Germ Layer Characterization Kit
Description:
Human Embryonic Germ Layer Characterization Kit
Trade Name:
Chemicon (Millipore)
Background Information:
Human embryonic stem cells (HESC) are pluripotent stem cells that are capable of self-renewal and of differentiation in vitro and in vivo into a wide variety of cell types, including derivatives of all three embryonic germ layers. These primary germ layers will eventually give rise to all the tissues and organs of the body.
Mesoderm: bone, cartilage, muscle, blood and vascular system
Endoderm: gastrointestinal tract, respiratory tract and endocrine glands (including liver and pancreas)
Ectoderm: nervous system, skin
CHEMICON®'s Human Embryonic Germ Layer Characterization Kit (SCR030) allows researchers to assess the differentiation potential of their HESC to form derivatives of all three embryonic germ layers. Included in the kit are antibodies that are routinely used by stem cell researchers to identify the lineage fates of HESC following either spontaneous or directed differentiation.
Mesoderm: Smooth Muscle Actin (CBL171)
Cardiac Troponin I (MAB1691-50UG)
Endoderm: Alpha Fetoprotein (2004189)
Ectoderm: Nestin (AB5922)
MAP-2 (MAB3418-50UG)
Mesoderm: bone, cartilage, muscle, blood and vascular system
Endoderm: gastrointestinal tract, respiratory tract and endocrine glands (including liver and pancreas)
Ectoderm: nervous system, skin
CHEMICON®'s Human Embryonic Germ Layer Characterization Kit (SCR030) allows researchers to assess the differentiation potential of their HESC to form derivatives of all three embryonic germ layers. Included in the kit are antibodies that are routinely used by stem cell researchers to identify the lineage fates of HESC following either spontaneous or directed differentiation.
Mesoderm: Smooth Muscle Actin (CBL171)
Cardiac Troponin I (MAB1691-50UG)
Endoderm: Alpha Fetoprotein (2004189)
Ectoderm: Nestin (AB5922)
MAP-2 (MAB3418-50UG)
Key Applications:
- Immunocytochemistry
- Stem Cell Culture
Species Reactivity:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Stem Cell Workflow Stage:
Characterization
Components:
- 50 µL of Alpha Fetoprotein mouse monoclonal 2004189
- 50 µg of Smooth Muscle Actin mouse monoclonal CBL171
- 50 µL of Nestin rabbit polyclonal AB5922
- 50 µg of Cardiac Troponin I mouse monoclonal MAB1691-50UG
- 50 µg of MAP-2 mouse monoclonal MAB3418-50UG
Kit Type:
Stem Cell Kits
Stem Cell Type:
Human Embryonic Stem Cells
Kit or Assay Type:
Stem Cell Kits
Storage Conditions:
Maintain components according to the individual data sheets. Antibodies to alpha fetoprotein (2004189) and nestin (AB5922) are to be stored at -20°C (SCR030 - Part 1). Antibodies to smooth muscle actin (CBL171), cardiac troponin I (MAB1691-50UG) and MAP-2 (MAB3418-50UG) are to be stored at 2 - 8°C (SCR030 - Part 2).