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Ordering Information
- : SCR044
- : 1 kit
Rat Neural Stem Cell Blotting Kit
Description:
Rat Neural Stem Cell Blotting Kit
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 kit
Product Overview:
Kit containing 2 blots, each containing cellular extracts from neural stem cells and antibodies for detection of signalling molecules involved in the maintenance and differentiation of neural stem cells. Cellular extracts on blots: 1) Neural stem cells isolated from the adult rat hippocampus 2) Neurons and oligodendrocytes differentiated from rat neural stem cells 3) Astrocytes differentiated from rat neural stem cells Other kit components: - 50 micrograms of Nestin monoclonal Ab - 100 microliters of GFAP monoclonal Ab - 50 microliters of Actin monoclonal Ab - 1 ReBlot Plus western Blot Recycling Kit - Trial
Key Applications:
Western Blotting
Species Reactivity:
Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
Characterization
Components:
- 2 protein blots containing normalized cellular extracts from: (Neural stem cells isolated from the adult rat hippocampus, Neurons and oligodendrocytes differentiated from rat neural stem cells, Astrocytes differentiated from rat neural stem cells)
- 50 μg of Nestin monoclonal (Part No. 2003602)
- 100 μL of GFAP monoclonal (Part No. MAB360)
- 50 μL of Actin monoclonal (Part No. 2004338)
- 1 ReBlot™ Plus Western Blot Recycling Kit - Trial (Part No. 2500-S)
Brand Family:
Chemicon
Storage Conditions:
Maintain unused blots at -20°C. Blots should be handled with care and should be used within 6 months from date of receipt.
GFAP and Actin monoclonal antibodies: Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze-thaw cycles.
Nestin monoclonal antibody: Maintain at 2-8°C in undiluted aliquots for up to six months after date of receipt.
ReBlotTM Plus Kit: Kit components should be stored at 2-8°C upon arrival. Product is stable for 3-6 months after receipt. If Antibody Stripping Solution crystallizes upon storage, it may be re-dissolved with gentle warming at 37°C before use. Note: To prevent reagent degradation, secure the cap tightly upon storage. Avoid extended exposure to air.
GFAP and Actin monoclonal antibodies: Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze-thaw cycles.
Nestin monoclonal antibody: Maintain at 2-8°C in undiluted aliquots for up to six months after date of receipt.
ReBlotTM Plus Kit: Kit components should be stored at 2-8°C upon arrival. Product is stable for 3-6 months after receipt. If Antibody Stripping Solution crystallizes upon storage, it may be re-dissolved with gentle warming at 37°C before use. Note: To prevent reagent degradation, secure the cap tightly upon storage. Avoid extended exposure to air.
Product Name:
Rat Neural Stem Cell Blotting Kit