Ordering Information

Catalogue Number : SCR045
Qty/Pk : 1 kit

Product Images

Immunocytochemistry staining of TGP52 cells, a mouse epithelial-like cell line derived from a pancreatic tumor with islet cell morphology (ATCC). In ...

Pancreatic Islet Cell Characterization Kit

Description:

Trade Name:

Chemicon (Millipore)

Qty/Pk:

1 kit

Product Overview:

The Pancreatic Islet Cell Characterization Kit (SCR045) provides a convenient set of validated antibodies that allows researchers to reliably identify mature pancreatic islets cells. Along with antibodies generated against discrete hormones secreted by alpha, beta, delta and gamma cells of the pancreatic islets, the kit includes PDX-1 (pancreatic duodenal homeobox gene-1), a master regulator of islet cell development and GLUT-2, a glucose transporter present in beta-islet cells. The pancreas functions as both an exocrine and endocrine organ. Exocrine functions include the secretion of digestive enzymes (trypsinogen, chymotrypsinogen, pancreatic lipase, and amylase) from pancreatic acinar cells into the gastrointestinal tract. Endocrine functions occur within small clusters of heterogeneous cells, called the islets of Langerhans that are dispersed throughout the pancreatic tissue. The pancreatic islets comprise specific cell populations (alpha, beta, delta and gamma cells) that secrete distinct hormones:

Alpha cells: Glucagon
Beta cells: Insulin / proinsulin (c-peptide)
Delta cells: Pancreatic polypeptide (PPP)
Gamma cells: Somatostatin

Diabetes results from the loss or dysfunction of insulin-producing beta-cells in the pancreas. Cell replacement therapies through islet transplantation is considered a potential long term approach to the control of blood glucose levels. However obtaining sufficient quantities of insulin-producing tissues for islet transplantation remains a major impediment. Stem cells (either from embryonic stem cells or from pancreatic progenitors) could potentially provide an abundant alternative source of islet cells for transplantation therapies.

Key Applications:

Immunocytochemistry

Application Notes:

For further information on any of the included antibodies, please see the individual antibody datasheets:
Rabbit anti-glucagon: Catalog No. AB932
Rabbit anti-somatostatin: Catalog No. AB5494
Mouse anti-pro-insulin C-peptide: Catalog No. CBL94
Rabbit anti-glut-2: Catalog No. 07-1402
Rabbit anti-PDX-1: Catalog No. AB3503
Rabbit anti-PPP: Catalog No. AB939

Species Reactivity:

Mouse

Usage Statement:

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Components: