Pancreatic Cell Development Pathway Kit
Description:
Pancreatic Cell Development Pathway Kit
Trade Name:
Chemicon (Millipore)
Product Overview:
The Pancreatic Cell Development Pathway Kit (SCR046) provides a collection of antibodies that are unique to key transition points along the developmental pathway of pancreatic cells. Included in the kit are antibodies to critical transcription factors expressed during the program of development along with two antibodies to hormones secreted by mature islets cells.
Background Information:
Diabetes results from the loss or dysfunction of insulin producing beta-cells in the pancreas. Cell replacement therapy through islet transplantation is considered a potential long term approach to the control of blood glucose levels. However obtaining sufficient quantities of insulin producing tissues for islet transplantation remains a major impediment. Stem cells (either from embryonic stem cells or from pancreatic progenitors) could potentially provide an abundant alternative source of islet cells for transplantation therapies. CHEMICON's Pancreatic Cell Development Pathway Kit (SCR046) provides a collection of antibodies that are unique to key transition points along the developmental pathway of pancreatic cells. Included in the kit are antibodies to critical transcription factors expressed during the program of development along with two antibodies to hormones secreted by mature islets cells.
First Transition: Early in development, (embryonic day 8.5 (e8.5) to e9.5 in the mouse), the pancreas originates from the endoderm as two buds that develop on the dorsal and ventral side of the foregut. From e10.5 to e12.5, the dorsal and ventral pancreatic buds undergo further transformation into two primitive pancreases with branching ducts and undifferentiated epithelium. Single endocrine cells are evident throughout the undifferentiated epithelium.
FoxA2
PDX-1
Hes-1
Second Transition: Differentiation of the buds into endocrine and exocrine cell lineages (e14). The dorsal and ventral primitive pancreases rotate and fuse to form the more developed pancreas (e13 to e15). Endocrine cells proliferate and become clustered as islets of Langerhans (e16 to e19).
PAX 6
Third Transition: Maturation of endocrine cells.
Glucagon
Pancreatic Polypeptide.
First Transition: Early in development, (embryonic day 8.5 (e8.5) to e9.5 in the mouse), the pancreas originates from the endoderm as two buds that develop on the dorsal and ventral side of the foregut. From e10.5 to e12.5, the dorsal and ventral pancreatic buds undergo further transformation into two primitive pancreases with branching ducts and undifferentiated epithelium. Single endocrine cells are evident throughout the undifferentiated epithelium.
FoxA2
PDX-1
Hes-1
Second Transition: Differentiation of the buds into endocrine and exocrine cell lineages (e14). The dorsal and ventral primitive pancreases rotate and fuse to form the more developed pancreas (e13 to e15). Endocrine cells proliferate and become clustered as islets of Langerhans (e16 to e19).
PAX 6
Third Transition: Maturation of endocrine cells.
Glucagon
Pancreatic Polypeptide.
Key Applications:
- Immunocytochemistry
- Stem Cell Culture
Species Reactivity:
- Human
- Mouse
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Stem Cell Workflow Stage:
Characterization
Components:
- 50 ug of FoxA2 rabbit polyclonal antibody
- 50 ug of Hes-1 rabbit polyclonal antibody
- 50 uL of Pax 6 rabbit polyclonal antibody
- 50 uL of IDX-1 (PDX-1), C-terminal rabbit polyclonal antibody
- 50 uL of Glucagon rabbit polyclonal antibody
- 50 uL of Pancreatic Polypeptide rabbit polyclonal antibody
Kit Type:
Stem Cell Kits
Stem Cell Type:
Pancreatic Stem Cells
Kit or Assay Type:
Stem Cell Kits
Storage Conditions:
Store all components at -20C