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MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit
Description:
MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit
Qty/Pk:
1 kit
Product Overview:
MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit provides ready-to use primary rat neural stem cells that are constitutively labeled with the humanized mulleri green fluorescent protein (hmGFP) along with expansion medium to help maintain expression of the transgene. hmGFP expression is driven off a constitutive promoter, chicken actin. These stable GFP transfected cells have been FACS sorted to over 95-100% purity.
The constitutive GFP Reporter Adult Rat Hippocampal NSCs display the immunochemical staining properties of NSC; they are Nestin+Sox2+TuJ1-GFAP-. These cells are multipotent. Under defined neuronal differentiation conditions (Rodent Neuron Differentiation Kit, Millipore Cat. No. SCR035), the majority of cells are differentiated to βIII-tubulin positive neurons (GFP+βIII-tubulin+GFAP-). Under defined astrocytic differentiation conditions (Astrocyte Differentiation Medium, Millipore Cat. No. SCM010), majority of cells differentiate to GFAP-positive astrocytes (GFP+GFAP+βIII-tubulin-).
The constitutive GFP Reporter Adult Rat Hippocampal NSCs display the immunochemical staining properties of NSC; they are Nestin+Sox2+TuJ1-GFAP-. These cells are multipotent. Under defined neuronal differentiation conditions (Rodent Neuron Differentiation Kit, Millipore Cat. No. SCR035), the majority of cells are differentiated to βIII-tubulin positive neurons (GFP+βIII-tubulin+GFAP-). Under defined astrocytic differentiation conditions (Astrocyte Differentiation Medium, Millipore Cat. No. SCM010), majority of cells differentiate to GFAP-positive astrocytes (GFP+GFAP+βIII-tubulin-).
Usage Statement:
For Research Use Only. EMD Millipore does not recommend that a user derive clones from cells provided with this kit. However, should a user decide to do so, they may need a license to one or more third party patents and it is incumbent upon that user to determine whether and if so which third party patents need to be licensed.
Quality Assurance:
Primary constitutive GFP reporter rat neural stem cells have been validated for high level of constitutive expression of GFP, high level of expression of Nestin and Sox 2, and for their self-renewal and multi-lineage differentiation capacities. Cells display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma.
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
- Cells/Isolation
- Maintenance & Expansion
Cell Line Type:
Neural Stem Cells
Components:
- MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells, 1 x 106 viable cells
- MilliTrace Rat Neural Stem Cell Expansion Medium (Cat. No. SCM040)
Storage Conditions:
· MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cells (Part No. SCC080) should be stored in Liquid Nitrogen. We recommend that the cells be used within ten passages.
· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until expiration date on the label. Upon thawing the basal medium should be stored at 2-8°C and given a 1-month expiration dating.
· 5 mg/mL Puromycin Solution (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.
· Lyophilized FGF-2 (Part No. GF003-10UG) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
· Neural Stem Cell Basal Medium (Part No. SCM003) should be stored at -20°C until expiration date on the label. Upon thawing the basal medium should be stored at 2-8°C and given a 1-month expiration dating.
· 5 mg/mL Puromycin Solution (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.
· Lyophilized FGF-2 (Part No. GF003-10UG) should be reconstituted with 100 µL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 µg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C for up to six months. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Multiple freeze/thaw cycles will result in significant loss of activity.
Cell Culture Reagent Type:
Stem Cell Reagents
Product Name:
MilliTrace Constitutive GFP Reporter Adult Rat Hippocampal Neural Stem Cell Kit