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MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit
Description:
MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit
Qty/Pk:
1 kit
Product Overview:
MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit provides ready-to-use mouse embryonic stem cells that are constitutively labeled with the humanized mulleri green fluorescent protein (hmGFP) along with expansion medium to help maintain expression of the transgene.
MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells were generated by transfection of C57/BL6 mouse embryonic stem cells with a proprietary bicistronic plasmid construct containing hmGFP under the control of a constitutive chicken actin promoter. FACS analyses of stable transfectants indicate that greater than 95% of the cells express GFP at high levels even after 10 passages. MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells display the immunochemical staining properties of pluripotent stem cells and are thus positive for alkaline phosphatase, Oct-4, Sox-2, and SSEA-1. Under neuronal differentiation conditions, cells can be differentiated to βIII-tubulin positive neurons (GFP+βIII-tubulin+) (Use Mouse Embryonic Stem Cell Neurogenesis Kit, Catalog No. SCR101).
MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells were generated by transfection of C57/BL6 mouse embryonic stem cells with a proprietary bicistronic plasmid construct containing hmGFP under the control of a constitutive chicken actin promoter. FACS analyses of stable transfectants indicate that greater than 95% of the cells express GFP at high levels even after 10 passages. MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells display the immunochemical staining properties of pluripotent stem cells and are thus positive for alkaline phosphatase, Oct-4, Sox-2, and SSEA-1. Under neuronal differentiation conditions, cells can be differentiated to βIII-tubulin positive neurons (GFP+βIII-tubulin+) (Use Mouse Embryonic Stem Cell Neurogenesis Kit, Catalog No. SCR101).
Usage Statement:
For Research Use Only. EMD Millipore does not recommend that a user derive clones from cells provided with this kit. However, should a user decide to do so, they may need a license to one or more third party patents and it is incumbent upon that user to determine whether and if so which third party patents need to be licensed.
Quality Assurance:
Constitutive GFP reporter mouse embryonic stem cells have been validated for high level constitutive expression of GFP, for high level of expression of alkaline phosphatase, Oct-4, Sox-2, and SSEA-1, and for their self-renewal and multi-lineage differentiation capacities. Cells display normal karyotype as assessed by G-banding of twenty metaphase cells and tested negative for mycoplasma.
Stem Cell Type:
Mouse Embryonic Stem Cells
Stem Cell Workflow Stage:
- Cells/Isolation
- Maintenance & Expansion
Cell Line Type:
Embryonic Stem Cells
Components:
- MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells, 1 x 106 viable cells
- MilliTrace Mouse Embryonic Stem Cell Expansion Medium (Catalog No. SCM042)
Storage Conditions:
· MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells (Part No. SCC082) should be stored in Liquid Nitrogen. We recommend that the cells be used within ten passages.
· EmbryoMax Complete ES Cell Medium with 15% FBS and LIF (Part No. ES-101-B) should be stored at -20°C until expiration date on the label. Upon thawing the medium should be stored at 2-8°C and given a 1-month expiration dating.
· 5 mg/mL Puromycin Solution (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.
· EmbryoMax Complete ES Cell Medium with 15% FBS and LIF (Part No. ES-101-B) should be stored at -20°C until expiration date on the label. Upon thawing the medium should be stored at 2-8°C and given a 1-month expiration dating.
· 5 mg/mL Puromycin Solution (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.
Product Name:
MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit