Mouse Embryonic Stem Cell Adipogenesis Kit
Description:
Mouse Embryonic Stem Cell Adipogenesis Kit
Product Overview:
The Mouse Embryonic Stem Cell Adipogenesis Kit provides a system designed for the differentiation of mouse ES cells into adipocytes. The kit contains all reagents necessary to differentiate mouse ES cells into Oil Red O positive adipocytes in vitro: (1) Gelatin Solution for proper support for cell attachment and spreading, (2) Accutase solution for the detachment of cells, (3) EB Formation Medium, a specially formulated medium that has been optimized and qualified to support the formation of embryoid bodies, (4) Inducer A Solution, (5) Insulin Solution and 3,3′,5-Triiodo-L-thyronine (T3), two hormones that aid in the differentiation of mouse ES cells to adipocytes, (6) Oil Red O Solution, a positive stain for adipocytes, and (7) Wash Solution and Dye Extraction Solution, both used to help quantify Oil Red O positive adipocytes using a spectrophotometer.
Background Information:
Adipocyte development has emerged as an attractive area of biomedical/ pharmaceutical research in recent years due to the dramatic increase in obesity and obesity related diseases (type-II diabetes, cardiovascular disease and cancers) worldwide. Current in vitro models of adipogenesis rely on freshly isolated adipose tissue, predetermined pre-adipocytes, mesenchymal stem cells or the 3T3-L1 cell line for in vitro assays. All of these models have drawbacks that include: timely tissue isolation, the inaccurate use of predetermined cells to study early development and scalability issues.
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and are capable of unlimited, undifferentiated proliferation in vitro under appropriate cell culture conditions. These cells have the unique ability to differentiate into cells comprising all three embryonic germ layers (ectoderm, mesoderm and endoderm). Because of their robust growth characteristics and pluripotent nature, embryonic stem cells have emerged as an attractive model to properly study the earliest stages of adipocyte development in vitro.
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and are capable of unlimited, undifferentiated proliferation in vitro under appropriate cell culture conditions. These cells have the unique ability to differentiate into cells comprising all three embryonic germ layers (ectoderm, mesoderm and endoderm). Because of their robust growth characteristics and pluripotent nature, embryonic stem cells have emerged as an attractive model to properly study the earliest stages of adipocyte development in vitro.
Key Applications:
Cell Differentiation
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Stem Cell Workflow Stage:
Differentiation
Components:
- Sufficient reagents are provided in the kit for 10 separate differentiation reactions.
- 1. Embryoid Body (EB) Formation Medium
- 2. Accutase Cell Dissociation Solution
- 3. Inducer A Solution
- 4. Insulin Solution
- 5. 3,3′,5-Triiodo-L-thyronine (T3) Solution
- 6. 0.1% Gelatin Solution
- 7. Oil Red O Solution
- 8. Wash Solution
- 9. Dye Extraction Solution
Kit Type:
Stem Cell Kits
Stem Cell Type:
Mouse Embryonic Stem Cells
Kit or Assay Type:
Stem Cell Kits
Storage Conditions:
Embryoid Body (EB) Formation Medium: Medium should be stored at -20°C until ready to use. At -20°C the medium is stable for up to six months. Medium is provided in convenient 100 mL bottles. Prior to initial use, thaw frozen media at 2 to 8°C overnight or until it has become completely equilibrated. Upon thawing, medium should be stored at 2 to 8°C and given a 1-month expiration dating.
Accutase: Stable when stored at -20°C. After thawing, Accutase may be stored for up to 2 months at 2 to 8°C. DO NOT STORE AT ROOM TEMP.
Inducer A Solution: Solution is light sensitive and is readily oxidized upon exposure to air. Inducer A Solution should be stored in working aliquots (5-10 µL) at -20°C for up to 1 year after date of receipt. Prolonged exposure to air and light will result in significant loss of activity. Only thaw aliquots that are required for the experiment. Upon thawing, discard any unused solutions.
Insulin and 3,3′,5-Triiodo-L-thyronine (T3) Solution: Solutions are stable for up to 1 year at -20°C. Avoid repeated freeze/thaw cycles.
0.1% Gelatin Solution: Solution is stable for up to 1 year at room temperature.
Oil Red O Solution, Wash Solution and Dye Extraction Solution: Solutions should be stored at room temperature for up to 1 year. Storage of Oil Red O Solution and Dye Extraction Solution at –20ºC may result in formation of insoluble precipitates and is not recommended. If Oil Red O solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.
Accutase: Stable when stored at -20°C. After thawing, Accutase may be stored for up to 2 months at 2 to 8°C. DO NOT STORE AT ROOM TEMP.
Inducer A Solution: Solution is light sensitive and is readily oxidized upon exposure to air. Inducer A Solution should be stored in working aliquots (5-10 µL) at -20°C for up to 1 year after date of receipt. Prolonged exposure to air and light will result in significant loss of activity. Only thaw aliquots that are required for the experiment. Upon thawing, discard any unused solutions.
Insulin and 3,3′,5-Triiodo-L-thyronine (T3) Solution: Solutions are stable for up to 1 year at -20°C. Avoid repeated freeze/thaw cycles.
0.1% Gelatin Solution: Solution is stable for up to 1 year at room temperature.
Oil Red O Solution, Wash Solution and Dye Extraction Solution: Solutions should be stored at room temperature for up to 1 year. Storage of Oil Red O Solution and Dye Extraction Solution at –20ºC may result in formation of insoluble precipitates and is not recommended. If Oil Red O solution forms a precipitate, remove particulates by passage through a 0.22 or 0.45-micron filter.