Mouse Embryonic Stem Cell Neurogenesis Kit
Description:
Mouse Embryonic Stem Cell Neurogenesis Kit
Product Overview:
The Mouse Embryonic Stem Cell Neurogenesis Kit provides a system designed for the neural differentiation of mouse ES cells. The kit contains all the reagents necessary to fully differentiate mouse ES cells into βIII-tubulin positive neurons in vitro following a modified 4-/4+ differentiation protocol (Bain, et al., 1995): (1) Poly-L-ornithine and Laminin, two key substrates that provide proper support for cell attachment and spreading, (2) Accutase Solution for the detachment of cells, (3) EB Formation Medium, a specially formulated medium that has been optimized and qualified to support the formation of embryoid bodies, (4) Neural Inducer A Solution, and (5) SSEA-1 and βIII-tubulin monoclonal antibodies to characterize the starting undifferentiated mouse ES cell populations and to validate the resulting neurons produced from the differentiation protocol provided, respectively.
Background Information:
Numerous neurological disorders, including Parkinson Disease, Alzheimer Disease, Amyotrophic Lateral Sclerosis (ALS) and spin cord injuries, remain inoperable disorders that are caused by an absence of functional neurons within the central nervous system. The expansion of functional neurons for transplantation and biomedical research remains inefficient.
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and are capable of unlimited, undifferentiated proliferation in vitro under appropriate cell culture conditions. These cells have the unique ability to differentiate into cells comprising all three embryonic germ layers (ectoderm, mesoderm and endoderm). Because of their pluripotent and robust growth characteristics, embryonic stem cells have been used to model neurogenesis and to generate large quantities of functional neurons for both in vitro and in vivo studies.
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and are capable of unlimited, undifferentiated proliferation in vitro under appropriate cell culture conditions. These cells have the unique ability to differentiate into cells comprising all three embryonic germ layers (ectoderm, mesoderm and endoderm). Because of their pluripotent and robust growth characteristics, embryonic stem cells have been used to model neurogenesis and to generate large quantities of functional neurons for both in vitro and in vivo studies.
Key Applications:
Cell Differentiation
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Stem Cell Workflow Stage:
Differentiation
Components:
- Sufficient reagents are provided in the kit for 10 separate differentiation reactions.
- 1. Embryoid Body (EB) Formation Medium
- 2. Accutase Cell Dissociation Soluton
- 3. Neural Inducer A Solution
- 4. Mouse Laminin (200 µg)
- 5. Poly-L-ornithine Solution
- 6. Mouse anti-SSEA-1 (50 µg)
- 7. Mouse anti-βIII tubulin (50 µg)
Kit Type:
Stem Cell Kits
Stem Cell Type:
Mouse Embryonic Stem Cells
Kit or Assay Type:
Stem Cell Kits
Storage Conditions:
Embryoid Body (EB) Formation Medium: Medium should be stored at -20°C until ready to use. At -20°C the medium is stable for up to six months. Medium is provided in convenient 100 mL bottles. Prior to initial use, thaw frozen media at 2 to 8°C overnight or until it has become completely equilibrated. Upon thawing, medium should be stored at 2 to 8°C and given a 1-month expiration dating.
Accutase: Stable when stored at -20°C. After thawing, Accutase may be stored for up to 2 months at 2 to 8°C. DO NOT STORE AT ROOM TEMP.
Neural Inducer A Solution: Solution is light sensitive and is readily oxidized upon exposure to air. Neural Inducer A Solution should be stored in working aliquots (10-20 µL) at -20°C for up to 1 year after date of receipt. Prolonged exposure to air and light will result in significant loss of activity.
Laminin Solution: Solution is stable for up to six months at -20°C.
Poly-L-ornithine Solution: Solution is stable for up to 2 years at -20°C.
Mouse anti-SSEA-1 and Mouse anti-βIII tubulin antibodies should be maintained at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Accutase: Stable when stored at -20°C. After thawing, Accutase may be stored for up to 2 months at 2 to 8°C. DO NOT STORE AT ROOM TEMP.
Neural Inducer A Solution: Solution is light sensitive and is readily oxidized upon exposure to air. Neural Inducer A Solution should be stored in working aliquots (10-20 µL) at -20°C for up to 1 year after date of receipt. Prolonged exposure to air and light will result in significant loss of activity.
Laminin Solution: Solution is stable for up to six months at -20°C.
Poly-L-ornithine Solution: Solution is stable for up to 2 years at -20°C.
Mouse anti-SSEA-1 and Mouse anti-βIII tubulin antibodies should be maintained at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.