Human Mesenchymal Stem Cell Kit
(Derived from Bone-Marrow)

Human Mesenchymal Stem Cell Kit
(Derived from Bone-Marrow)

Chemicon (Millipore)

Stem Cell Culture

Mesenchymal stem cells, also known as marrow stromal cells (1), are defined as a self-renewing population of adherent, bone-marrow-derived multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages. In defined in vitro assays, mesenchymal stem cells have been shown to readily differentiate into lineage-specific cells that form bone, cartilage, fat, tendon, and muscle tissues (1, 2). Mesenchymal stem cells also provide support and maintenance for the other major stem cell population in the bone marrow, the hematopoietic stem cells (2).
Mesenchymal stem cells (MSC’s) have historically been isolated based on the ability of these cells to form adherent cell layers in culture and the concomitant lack of adherence of other cells in the bone marrow stroma such as hematopoietic stem cells, adipocytes, and macrophage (1, 3). While this procedure results in enriched populations of mesenchymal stem cells, the resulting bone-marrow derived cell populations are, nonetheless, heterogeneous – comprised not only of mesenchymal stem cells, but also of committed lineage-restricted progenitors (1, 3). To aid researchers in the accurate identification and characterization of mesenchymal stem cells, Millipore provides cryopreserved Human Mesenchymal Stem Cells and optimized media in this kit. Millipore’s cryopreserved Human Mesenchymal Stem Cells are ready-to-use primary mesenchymal stem cells isolated from the iliac crest of normal human bone marrow (18-30 years old).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Millipore’s Human Mesenchymal Stem Cells are derived from human bone marrow and have been validated for high expression level of cell surface molecules that are present on mesenchymal stem cells: H-CAM (CD44), THY-1 (CD90) and STRO-1 and for their absence of hematopoietic cell surface markers, CD14 (present on leukocytes) and CD19 (present on B-lymphocytes). Millipore’s Human Mesenchymal Stem Cells are also negative for the endothelial marker, M-CAM (CD146). The cells have also been validated for their self-renewal and multi-lineage differentiation capacities (please refer to product manual figures for representative data). Cells display normal karyotype as assessed by G-banding of 20 metaphase cells and tested negative for mycoplasma. Each lot of Mesenchymal Stem Cell medium is tested for the optimal cell growth and proliferation of mesenchymal stem cells. Sterility Testing: Negative

Mesenchymal Stem Cell Culture Media

Mesenchymal Stem Cells

• Maintenance & Expansion
• Cells/Isolation

Cell Culture Media

Human bone marrow

Human Mesenchymal Stem Cells (Part # SCC034) should be stored in liquid nitrogen. It is recommended that the cells be used within ten passages.
Mesenchymal Stem Cell Expansion Medium: (Catalog No. SCM015) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.

Cell Culture Media