Human STEMCCA Cre-Excisable Constitutive Polycistronic (OKS/L-Myc) Lentivirus Reprogramming Kit

Human STEMCCA Cre-Excisable Constitutive Polycistronic (OKS/L-Myc) Lentivirus Reprogramming Kit

1kit

EMD Millipore’s Human STEMCCA Cre-Excisable Constitutive Polycistronic (OKS/L-Myc) Lentivirus Kit contains high titer Cre-excisable polycistronic (OKS/L-Myc) lentivirus, three small molecule rerogramming supplements, and Polybrene® transfection reagent. Efficient reprogramming of human somatic cells is achieved with significantly less virus required (20 MOI) and each lot of virus is functionally validated to form human iPS cells from EMD Millipore’s FibroGRO Xeno-Free Human Foreskin Fibroblasts (HFFs). Human iPS cells displayed characteristic ES cell-like morphology, stained positive for alkaline phosphatase, expressed the correct human pluripotent markers (Oct4, SSEA-4, TRA-1-60 and TRA-1-81) and can be expanded in normal human ES cell culture conditions. The use of L-Myc in the context of an excisable single lentiviral vector instead of four separate vectors for the derivation of iPS cells significantly reduces the risks of transformation, insertional mutagenesis and viral reactivation and is a step towards safer utilization of iPS technology for disease models and clinical therapies.

• This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
• Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

EMD Millipore’s human STEMCCA lentivirus has been tested to confirm the generation of iPS cells from p6 human foreskin fibroblasts. Other cell types have not been tested and thus similar results can not be guaranteed.

• 1. EF1a-hSTEMCCA-LoxP (OKS/L-Myc) Lentivirus: (Part No. CS207826): One (1) vial containing 15 μL of high titer lentivirus.
• 2. TGF-β RI Kinase Inhibitor IV Supplement (1000X): (Part No. CS210445): One (1) vial containing 400 μL of the inhibitor in high quality DMSO.
• 3. Sodium Butyrate Supplement (1000X): (Part No. CS210446): One (1) vial containing 400 μL of the inhibitor in water.
• 4. PS48 Supplement (1000X): (Part No. CS210447): One (1) vial containing 400 μL of the inhibitor in high quality DMSO.
• 5. Polybrene 10 mg/mL: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock of Polybrene transfection reagent.

Lentivirus is stable for at least 6 months when stored at -80°C. After first thaw, place immediately on ice and store in working aliquots to avoid further freeze thaws. Avoid freeze thaws as this will result in a decrease in the virus titer. Polybrene transfection reagent is stable for at least 1 year when stored at -20°C.

Human STEMCCA Cre-Excisable Constitutive Polycistronic (OKS/L-Myc) Lentivirus Reprogramming Kit