MILLIPLEX MAP Human Skin Panel

MILLIPLEX MAP Human Skin Panel

MILLIPLEX

96-well plate

Millipore’s MILLIPLEX MAP Human Skin Panel has been expanded and can be used for the simultaneous quantitation of any or all of the following seven human skin biomarkers: fibronectin, cortisol, keratin-6, involucrin, keratin 1,10,11 and now human serum albumin (HSA) and lipopolysaccharide (LPS). More sensitive than clinical observation in its ability to detect subtle, treatment-induced changes in SC structural integrity, the Human Skin Panel is able to analyze analyte concentrations in skin cell extracts or skin samples collected with a non-invasive tape absorption technique.

Skin is the largest organ in the body, with such diverse functions as mechanical barrier, thermoregulation, vitamin D synthesis, vitamin B folate protection, fluid homeostasis, sensation, and innate immunity. The skin responds to damage by up-regulating repair mechanisms and causing skin hyperproliferation. Consequently, analysis of biochemical components from the stratum corneum (SC) can be used to evaluate skin irritation, SC integrity and structure and inflammatory changes in human skin.

Multiplexing

25 µL sample volume. Overnight assay 25 µL sample volume, skintape extract, skin biopsy extract, or skin culture lysate

Human

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Design your multiplex kit by choosing from 7 analytes.

Contact Technical Service for this value

• Fibronectin 0.03 ng/mL
• Cortisol 0.86 ng/mL
• Keratin-6 2.10 ng/mL
• Keratin-1,10,11 0.46 ng/mL
• Involucrin 0.004 ng/mL
• HSA 0.35 ng/mL
• LPS 0.025 ng/mL

Skin

Recommended storage for kit components is 2 - 8°C.

• Inter-assay: 6.5 – 13.6
• Intra-assay: 2.3 – 6.0

70 – 111%

• Fluorescent
• Luminex xMAP

Antibody Arrays

The antibody pairs in the panel are specific only to the desired analyte and exhibit no or negligible cross-reactivity with other analytes in the panel.

MILLIPLEX MAP Human Skin Panel

• Fibronectin 0.06–250 ng/mL
• Cortisol 0.12–500 ng/mL
• Keratin-6 0.64–2600 ng/mL
• Keratin-1, 10, 11 lot variable - refer to analysis sheet for exact concentration
• Involucrin 0.01–50 ng/mL
• HSA 0.21 - 850 ng/mL
• LPS 0.06 - 250 ng/mL

Cortisol, Fibronectin, HSA, Involucrin, Keratin-1,10,11, Keratin-6, LPS