Content Loading...
Content Loading...
Ready-to-Screen Tissue BLOTS™ Mouse Testis
Description:
Ready-to-Screen Tissue BLOTS™ Mouse Testis
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 blot
Product Overview:
Tissue blot from Mouse Testis
ORIENTATION:
Lane 1 - Molecular Weight Marker (250 kDa, 148 kDa, 60 kDa, 42 kDa,
30 kDa, 22 kDa, 17 kDa)
Lane 2 - Mouse Testis
GEL TYPE:
15% Polyacrylamide, Gel Thickness: 1.5 mm
ELECTRODE BUFFER:
Tris-Glycine, Electrophoresis Time: 2 hrs, Electroblotting Time: 2 hrs
PROTEIN STANDARDS:
Pre-stained Markers (Novex, Inc.)
ORIENTATION:
Lane 1 - Molecular Weight Marker (250 kDa, 148 kDa, 60 kDa, 42 kDa,
30 kDa, 22 kDa, 17 kDa)
Lane 2 - Mouse Testis
GEL TYPE:
15% Polyacrylamide, Gel Thickness: 1.5 mm
ELECTRODE BUFFER:
Tris-Glycine, Electrophoresis Time: 2 hrs, Electroblotting Time: 2 hrs
PROTEIN STANDARDS:
Pre-stained Markers (Novex, Inc.)
Key Applications:
Western Blotting
Application Notes:
INSTRUCTION FOR USE:
The blot is stored in TBST buffer. Do not let blot dry at any step. Always wear gloves when handling the blots. If the blot is not used immediately place the blot at 2-8°C. When needed remove the blot from the plastic bag. Place the membrane directly in a tray and proceed with desired blocking step. Incubate the membrane with primary and secondary antibody. Use chemiluminescent or color development reagents for the detection of the specific protein.
The blot is stored in TBST buffer. Do not let blot dry at any step. Always wear gloves when handling the blots. If the blot is not used immediately place the blot at 2-8°C. When needed remove the blot from the plastic bag. Place the membrane directly in a tray and proceed with desired blocking step. Incubate the membrane with primary and secondary antibody. Use chemiluminescent or color development reagents for the detection of the specific protein.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Blotting Membrane Type:
Preblotted Membranes
Quality Assurance:
The protein was isolated from mouse testis tissue by preparing a tissue homogenate in the presence of protease inhibitors. 75 μg of protein was solubilized in SDS-lysis buffer, electrophoresed in 15% SDS-PAGE, followed by electroblotting on PVDF membrane. Actin antibody was used to test the separation and transfer of proteins on the membrane.
Brand Family:
Chemicon
Storage Conditions:
Maintain at 2-8°C.
Product Name:
Ready-to-Screen Tissue BLOTS™ Mouse Testis