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Ready-to-Screen Tissue BLOTS™ Human Lung
Description:
Ready-to-Screen Tissue BLOTS™ Human Lung
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 blot
Product Overview:
Tissue blot from Human Lung
Key Applications:
Western Blotting
Application Notes:
INSTRUCTION FOR USE:
The blot is stored soaked in TBST buffer. Do not let blot dry at any step. Always wear gloves when handling the blots. Remove the blot from the plastic bag. Place the membrane directly in a tray containing desired blocking buffer and incubate for 30 minutes to 2 hours. Incubate the membrane with primary and secondary antibody. Use chemiluminescent or color development reagents for the detection of the specific protein.
ORIENTATION:
Lane 1: Molecular weight markers (top to bottom): 250 kDa (blue), 148 kDa (brown), 60 kDa (pink), 42 kDa (pink), 30 kDa (purple), 22 kDa (pink), 17 kDa (yellow) and 6 kDa* (pink).
*Due to low molecular weight it migrates almost along with sample dye and during transfer it diffuses, therefore you may not be able to see this marker on the membrane.
Lane 2: Human lung
The blot is stored soaked in TBST buffer. Do not let blot dry at any step. Always wear gloves when handling the blots. Remove the blot from the plastic bag. Place the membrane directly in a tray containing desired blocking buffer and incubate for 30 minutes to 2 hours. Incubate the membrane with primary and secondary antibody. Use chemiluminescent or color development reagents for the detection of the specific protein.
ORIENTATION:
Lane 1: Molecular weight markers (top to bottom): 250 kDa (blue), 148 kDa (brown), 60 kDa (pink), 42 kDa (pink), 30 kDa (purple), 22 kDa (pink), 17 kDa (yellow) and 6 kDa* (pink).
*Due to low molecular weight it migrates almost along with sample dye and during transfer it diffuses, therefore you may not be able to see this marker on the membrane.
Lane 2: Human lung
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Blotting Membrane Type:
Preblotted Membranes
Quality Assurance:
The protein was isolated from human lung by preparing a tissue homogenate in the presence of protease inhibitors. 75 μg of protein was solubilized in 2x SDS-lysis buffer, electrophoresed on 4-20% polyacrylamide gel, followed by electroblotting on PVDF membrane. The blot was stained with Ponceau S-Red to test the efficiency of the transfer of proteins. Gel Thickness: 1.5 nm, Electrode Buffer: Tris-Glycine, Electrophoresis Time: 4 hrs, Electroblotting time: 8 hrs.
Brand Family:
Chemicon
Storage Conditions:
Maintain at 2-8°C for up to four months after date of receipt.
Product Name:
Ready-to-Screen Tissue BLOTS™ Human Lung