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Ready-to-Screen Tissue BLOTS™ Human Multiple - Brain, Heart, Liver, Lung, Kidney, Placenta, Pancreas, Ovary, and Testis
Description:
Ready-to-Screen Tissue BLOTS™ Human Multiple - Brain, Heart, Liver, Lung, Kidney, Placenta, Pancreas, Ovary, and Testis
Trade Name:
Chemicon (Millipore)
Qty/Pk:
1 blot
Product Overview:
Tissue blot from human brain, heart, liver, lung, kidney, placenta, pancreas, ovary, and testis. Note: Source material has been tested and found to be free from HIV and Hepatitis by PCR.
Orientation: Orient blot with colored marker lane on left; blue marker band at top.
Lane 1 is colored standards: 180 kDa (blue); 115 kDa (blue); 82 kDa (blue); 64 kDa (pink); 49 kDa (blue); 37 kDa (blue); 26 kDa (blue); 19 kDa* (blue); 16 kDa (blue); 6 kDa* (blue).
Lane 2: Brain
Lane 3: Heart
Lane 4: Liver
Lane 5: Lung
Lane 6: Kidney
Lane 7: Placenta
Lane 8: Ovary
Lane 9: Testis
*Due to low molecular weight it migrates almost along with sample dye and during transfer it diffuses, therefore you may not be able to see this marker on the membrane.
Orientation: Orient blot with colored marker lane on left; blue marker band at top.
Lane 1 is colored standards: 180 kDa (blue); 115 kDa (blue); 82 kDa (blue); 64 kDa (pink); 49 kDa (blue); 37 kDa (blue); 26 kDa (blue); 19 kDa* (blue); 16 kDa (blue); 6 kDa* (blue).
Lane 2: Brain
Lane 3: Heart
Lane 4: Liver
Lane 5: Lung
Lane 6: Kidney
Lane 7: Placenta
Lane 8: Ovary
Lane 9: Testis
*Due to low molecular weight it migrates almost along with sample dye and during transfer it diffuses, therefore you may not be able to see this marker on the membrane.
Key Applications:
Western Blotting
Application Notes:
INSTRUCTIONFOR USE:
The blot is stored soaked in TBST buffer. Do not let the blot dry at any step. Always wear gloves when handling the blots. If the blot is not used immediately store the blot at 2-8°C. When needed remove the blot from the plastic bag. Place the membrane directly in a tray containing desired blocking buffer. Incubate the membrane with primary and secondary antibody. Use chemiluminescent or color development reagents for the detection of the specific protein.
The blot is stored soaked in TBST buffer. Do not let the blot dry at any step. Always wear gloves when handling the blots. If the blot is not used immediately store the blot at 2-8°C. When needed remove the blot from the plastic bag. Place the membrane directly in a tray containing desired blocking buffer. Incubate the membrane with primary and secondary antibody. Use chemiluminescent or color development reagents for the detection of the specific protein.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Blotting Membrane Type:
Preblotted Membranes
Quality Assurance:
The protein was isolated from various tissues by preparing a tissue homogenate in the presence of protease inhibitors. 75 μg of protein was solubilized in SDS-lysis buffer, electrophoresed on 4-20% polyacrylamide gel, followed by electroblotting on PVDF membrane. Actin antibody was used to test the separation and transfer of proteins on the membrane. Gel Thickness: 1.5 mm, Electrode Buffer: Tris-Glycine, Electrophoresis Time: 4 hrs, Electroblotting time: Overnight.
Brand Family:
Chemicon
Storage Conditions:
Maintain at 2-8°C for up to four months after receipt.
Product Name:
Ready-to-Screen Tissue BLOTS™ Human Multiple - Brain, Heart, Liver, Lung, Kidney, Placenta, Pancreas, Ovary, and Testis