Amicon Ultra centrifugal filter units are the first devices to provide both ultra-fast spin times and ultra-high recoveries (typically >90%). The vertical membrane reduces concentration polarization for spin times as fast as 5–10 minutes. The heat-sealed membrane minimizes downstream extractables. The units are 100% integrity tested for reliable performance.
Swinging bucket rotor: 4,000 x g for dilute samples; 3,500 x g for concentrated or viscous solutions. Fixed angle rotor: 7,500 x g for 3, 10, 30, and 50K; 5,000 x g for 100K
What pipette or pipette tips do you recommend to recover the retentate from the Amicon Ultra concentrators?
» Answer
We do not have any recommendation for pipette or pipette tip in the insert-booklet of Amicon Ultra. Certain pipettes, like Eppendorf 100-1000µl, are very wide and the tip can not reach the bottom of the device. So a smaller pipette is needed. The pipette we use for all the studies is a Gilson pipetman with regular pipette tips 1-200µl from Fisher cat# 21-197-2J.
Can the Amicon Ultra be used with nucleic acids?
» Answer
Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide.
What is the recommended rotor for Amicon Ultra units?
» Answer
Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units.