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Ordering Information

  • : XL-85K
  • : 250 tubes

Multi-Species Leptin RIA

Description:
Multi-Species Leptin RIA
Qty/Pk:
250 tubes
Background Information:
Leptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
Applications:
This radioimmunoassay (RIA) measures & quantifies Leptin for use in Metabolism & Endocrine & Neuroscience research.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium incubationat 4°C. Sample volume 100 µL serum, plasma, or tissue culture media
Species Reactivity:
  • Human
  • Mouse
  • Rat
  • Pig
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Linearity of Dilution:
94–115%
Label:
125I-Mouse Leptin
Standard Curve Range:
1–50 ng/mL (Human equivalent)
Product Name:
Multi-Species Leptin RIA
Specificity:
Human Leptin 100% Porcine Leptin 67% Rat Leptin 61% Mouse Leptin 73% Human Insulin Not Detectable Human Proinsulin Not Detectable Human C-Peptide Not Detectable Rat Insulin Not Detectable Glucagon Not Detectable IGF-I Not Detectable Canine Lepti
Analytes Available:
Leptin
Precision, %:
  • Inter-assay: 6.5–8.7
  • Intra-assay: 2.8–3.6
Detection Methods:
  • Radioactive
  • 125I
Accuracy:
83–104%
Performance:
  • ED80 = 3.0 ± 0.28 ng/mL HE
  • ED50 = 8.1 ± 0.60 ng/mL HE
  • ED20 = 24.4 ± 2.0 ng/mL HE, HE = Human Equivalent

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