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Poly-D-Lysine solution, 1.0 mg/mL
Description:
Poly-D-Lysine solution, 1.0 mg/mL
Qty/Pk:
20 mL
Product Overview:
Poly-D-Lysine promotes the adhesion of tissues/sections to the culture vessel. The polycationic property of the molecule allows interaction with anionic sites on the tissue/cells promoting effective attachment to the growing surface. The molecular weight of Poly-D-Lysine is >300,000 Da.
Key Applications:
Cell Culture
Application Notes:
The level of coating will vary for different species and types of cells. In general final coating concentrations from 3-10μg/cm(2) of surface area will typically supply sufficent coatings for most cell types.
Surface area of plates: (pi)(Radius squared) in cm(2)
In partical terms working dilutions of 50μg/mL to 100μg/mL are usually adequate.
Dilute poly-D-lysine in PBS, water, or 0.1M borate buffer {prepared by adding 1.24 g boric acid and 1.9 g sodium tetraborate in 400 ml water, pH 8.5} to final working concentration.
Plates or flasks are coated for 3 hours to overnight, and up to 3 days if stored at 4C. Best results are usually with just over night coatings.
Remove poly-d-lysine solution and rinse with sterile PBS or water 1-2X. Do not scrape the bottom of the coated plates or flasks.
Use plates immediately or store with PBS for up to 5 days.
Poly-D-lysine coating methods will vary slightly from laboratory to laboratory, please use the method as a general guide.
Surface area of plates: (pi)(Radius squared) in cm(2)
In partical terms working dilutions of 50μg/mL to 100μg/mL are usually adequate.
Dilute poly-D-lysine in PBS, water, or 0.1M borate buffer {prepared by adding 1.24 g boric acid and 1.9 g sodium tetraborate in 400 ml water, pH 8.5} to final working concentration.
Plates or flasks are coated for 3 hours to overnight, and up to 3 days if stored at 4C. Best results are usually with just over night coatings.
Remove poly-d-lysine solution and rinse with sterile PBS or water 1-2X. Do not scrape the bottom of the coated plates or flasks.
Use plates immediately or store with PBS for up to 5 days.
Poly-D-lysine coating methods will vary slightly from laboratory to laboratory, please use the method as a general guide.
Species Reactivity:
All
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Media or Reagent Type:
Attachment Factors
Stem Cell Type:
- Human Embryonic Stem Cells
- Mesenchymal Stem Cells
- Neural Stem Cells
- Hematopoietic Stem Cells
- Epithelial Cells
- Pancreatic Stem Cells
- Induced Pluripotent Stem Cells
Stem Cell Workflow Stage:
Maintenance & Expansion
Brand Family:
Specialty Media
Presentation:
Liquid. 20ml in sterile water.
Storage Conditions:
Store at -20C for upto 18 months.
Cell Culture Reagent Type:
- Stem Cell Reagents
- Attachment Factors
Product Name:
Poly-D-Lysine solution, 1.0 mg/mL
Alternate Names:
poly lysine