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Western Blot Analysis:
Representative lot data.
Mouse brain lysate was probed with Anti-PACSIN3 using a 1:250 dilution. Proteins were visual...
Anti-PACSIN3, N-terminus
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H | WB, IH(P) | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-PACSIN3, N-terminus
Specificity:
The antibody recognizes human and mouse PACSIN3.
Molecular Weight:
~53 kDa
Epitope:
N-terminus
Immunogen:
KLH conjugated synthetic peptide selected from the N-terminal region of human PACSIN3.
Background Information:
PACSINs are a protein family consisting of three members that have been implicated in synaptic vesicular membrane trafficking and regulation of dynamin-mediated endocytotic processes (Cuajungco, 2006). It is presumed that all isoforms bind Dynamin, Synaptojanin 1 and N-WASP through their Src homology 3 domains. While PACSIN1 appears to be restricted to neural tissues, two PACSINs are more widespread with PACSIN2 being ubiquitously expressed and PACSIN3 present mainly in skeletal muscle, heart and lung (Modregger, 2001). PACSIN3 (protein kinase C and casein kinase substrate in neurons 3) contains a short proline-rich region and lacks asparagine-proline-phenylalanine motifs, that differentiates it from the rest of the PACSIN family. Expected molcular weight is 49 kDa.
Species Reactivity:
- Mouse
- Human
Species Reactivity Note:
Human and mouse. Reactivity with other species has not been tested.
Control:
Mouse brain lysate
Quality Assurance:
Evaluated on a representative lot by Western blot on mouse brain lysate using 1:250 dilution of Anti-PACSIN3.
Purification Method:
Protein G purfied
Presentation:
PBS containing 0.09% NaN3.
Storage Conditions:
Maintain at 2-8ºC for up to 6 months from date of receipt. -20ºC is recommended for long term storage.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
SDPIII
Alternate Names:
- Endophilin I
- SH3 domain-containing protein 6511
- protein kinase C and casein kinase substrate in neurons 3
- syndapin III
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: May play a role in vesicle formation and transport.
SUBUNIT STRUCTURE: Homo- and hetero-aggregates with other PACSINs. Binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). Also interacts with MDC9 and MDC15 By similarity.
SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note= Detected at the inner aspect of the plasma membrane in cells or myotubes.
TISSUE SPECIFICTY: Expressed in heart and skeletal muscle, lung, kidney, adrenal gland and placenta.
PTM: Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC) Probable.
SEQUENCE SIMILARITIES: Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain.
SUBUNIT STRUCTURE: Homo- and hetero-aggregates with other PACSINs. Binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). Also interacts with MDC9 and MDC15 By similarity.
SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note= Detected at the inner aspect of the plasma membrane in cells or myotubes.
TISSUE SPECIFICTY: Expressed in heart and skeletal muscle, lung, kidney, adrenal gland and placenta.
PTM: Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC) Probable.
SEQUENCE SIMILARITIES: Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain.
Qty/Pk:
100 µg
Format:
Purified
Host:
Rabbit
Product Name:
Anti-PACSIN3, N-terminus
Concentration:
0.25 mg/mL
Antibody Type:
Polyclonal Antibody