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Goat anti-ChAT (Catalogue No. AB144P) staining of organotypic slice cultures of septum from 7-day-old rat tissue maintained in culture for 8 days.
Coronal section of rat neocortex stained with catalog number AB144P showing the choline acetyltransferase positive cholinergic nerve fibers and termin...
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Anti-Choline Acetyltransferase
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, Mk, Op, Av, Ch, Gp, Zebrafish | IH(P), IC, IH, WB | Goat | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Choline Acetyltransferase
Alternate Description:
Choline Acetyltransferase Antibody
Trade Name:
Chemicon (Millipore)
Product Overview:
Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
Specificity:
Choline acetyltransferase found in cholinergic neurons in brain and spinal cord.
Molecular Weight:
70/74 kDa
Immunogen:
Human placental enzyme.
Background Information:
Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker.
Species Reactivity:
- Human
- Rat
- Mouse
- Monkey
- Opossum
- Avian
- Chicken
- Guinea Pig
- Zebrafish
Species Reactivity Note:
Human and mouse. Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.
Demonstrated to react in zebrafish in THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).
Demonstrated to react in zebrafish in THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).
Application Notes:
Immunohistochemistry:
A previous lot of this antibody was used at 1:100.
Optimal working dilutions and protocols must be determined by end user.
A previous lot of this antibody was used at 1:100.
Optimal working dilutions and protocols must be determined by end user.
Control:
Human placenta lysates, rat forebrain/rostral hypothalamus.
Quality Assurance:
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.
Purification Method:
Affinity Purfied
Presentation:
Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
For short term storage (3-4 weeks) maintain at 2-8°C.
For short term storage (3-4 weeks) maintain at 2-8°C.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CHAT
- ChAT
- CHOACTase
- CMS1A
- CMS1A2
Alternate Names:
- Choline acetylase
- acetyl CoA:choline O-acetyltransferase
- choline acetyltransferase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry (Paraffin)
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
Entrez Gene Summary:
Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
UniProt Summary:
FUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
Brand Family:
Chemicon
Product Name:
Anti-Choline Acetyltransferase
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
500 µL
Format:
Affinity Purified
Host:
Goat