Ordering Information
- : AB152
- : 100 µL
Product Images
Rabbit anti-Tyrosine Hydroxylase (Catalog Number AB152) localization of tyrosine hydroxylase in culture of primary neurons. Photo courtesy of Dr. Mehd...
anti-Tyrosine Hydroxylase (TH) staining in mouse primary neural cultures using AB152 shown with an FITC fluorescent secondary (green). Fixation was 4...
see more > localization of tyrosine hydroxylase in culture of primary neurons. Photo courtesy of Dr. Mehdi Doroudchi, Avigen.'))
 staining in mouse primary neural cultures using AB152 shown with an FITC fluorescent secondary (green). Fixation was 4% PFA and the primary antibody incubation was 1:1000, overnight at 4°C.'))
 labelled mouse primary neural cultures using AB152 and a FITC labelled secondary (green). Fixation was 4% PFA and the primary antibody incubation was 1:1000, overnight at 4°C.'))
 staining of rat brain stem.'))
 staining on normal substantia nigra. Tissue pretreated with Citrate, pH 6.0 antigen retrieval. Polyclonal Ab. diluted to 1:300, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern to include cell body and synaptic nerve terminals of dopaminergic & noradrenergic neurons. Low Mag.'))
 staining on normal substantia nigra. Tissue pretreated with Citrate, pH 6.0 antigen retrieval. Polyclonal Ab. diluted to 1:300, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern to include cell body and synaptic nerve terminals of dopaminergic & noradrenergic neurons. High Mag.'))
. <br />Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.<br /><br />Arrow indicates protein tyrosine hydroxylase (~62 kDa).'))
Anti-Tyrosine Hydroxylase Antibody | AB152
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M, Fe, Ft, Sqd, Dr, Ml | ELISA, IF, IHC, IH(P), IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Tyrosine Hydroxylase Antibody | AB152
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Tyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
Molecular Weight:
62 kDa
Immunogen:
Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
Background Information:
Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
Species Reactivity:
- Human
- Rat
- Mouse
- Feline
- Ferret
- Squid
- Drosophila
- Mollusk
Species Reactivity Note:
Rat, mouse, ferret, feline, Aplysia (marine mollusc). It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
Application Notes:
Immunohistochemistry:
A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.
Immunofluorescence:
A previous lot of this antibody was used at 1:100-1:1,000 dilution.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
ELISA:
A previous lot of this antibody was used in ELISA.
Optimal dilutions must be determined by the end user.
A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.
Immunofluorescence:
A previous lot of this antibody was used at 1:100-1:1,000 dilution.
Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.
ELISA:
A previous lot of this antibody was used in ELISA.
Optimal dilutions must be determined by the end user.
Control:
POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
NEGATATIVE CONTROL: Liver.
NEGATATIVE CONTROL: Liver.
Quality Assurance:
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.
Purification Method:
Affinity Purfied
Presentation:
Purified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.
Storage Conditions:
Maintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- TH
- TYH
- EC 1.14.16.2
Alternate Names:
- Tyrosine 3-hydroxylase
- Tyrosine 3-hydroxylase
- tyrosine hydroxylase
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunofluorescence
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
- Western Blotting
Entrez Gene Summary:
Tyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
UniProt Summary:
FUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
COFACTOR: Fe(2+) ion.
SIZE: 528 amino acids; 58524 Da
TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Brand Family:
Chemicon
Product Name:
Anti-Tyrosine Hydroxylase Antibody | AB152
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µL
Format:
Affinity Purified
Host:
Rabbit