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Ordering Information
- : AB17003
- : 100 μg
- : 1 mg/mL
Product Images
Western blot analysis of Bim in K562 (K) and A549 (A) whole cell lysates with anti-Bim (IN) at 1:1000 dilution.
Anti-Bim, internal epitope, pan-Bim isoforms
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-Bim, internal epitope, pan-Bim isoforms
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes pan-Bim isoforms.
Molecular Weight:
23 kDa
Epitope:
internal epitope, pan-Bim isoforms
Immunogen:
Synthetic peptide corresponding to amino acids 22-40 of human origin (O'Conneor et al. 1998). The sequence is identical to that of mouse and is different by one amino acid from that of rat.
Species Reactivity:
- Human
- Mouse
- Rat
Application Notes:
Western blot: 1:1000-1:2000
Human K562 cell lysate can be used as a positive control and a 23 kDa band can be detected.
Optimal working dilutions must be determined by end user.
Human K562 cell lysate can be used as a positive control and a 23 kDa band can be detected.
Optimal working dilutions must be determined by end user.
Control:
Skin/Skin Tumor
Purification Method:
Immunoaffinity purified
Presentation:
Immunoaffinity Purified immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Storage Conditions:
The undiluted antibody solution is stable for approximately 6 months when stored 2–8°C.
UniProt Number:
Alternate Names:
BOD
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
UniProt Summary:
Function: Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
Subunit structure: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK By similarity.
Subcellular location: Endomembrane system; Peripheral membrane protein By similarity. Note: Associated with intracytoplasmic membranes By similarity.
Isoform BimEL: Mitochondrion
Isoform BimL: Mitochondrion
Isoform BimS: Mitochondrion
Isoform Bim-alpha1: Mitochondrion
Tissue specificity: Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Ref.3
Domain: The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Sequence similarities: Belongs to the Bcl-2 family.
Subunit structure: Forms heterodimers with a number of antiapoptotic Bcl-2 proteins including MCL1, BCL2, BCL2L1 isoform Bcl-X(L), BCL2A1/BFL-1, and BHRF1. Does not heterodimerize with proapoptotic proteins such as BAD, BOK, BAX or BAK By similarity.
Subcellular location: Endomembrane system; Peripheral membrane protein By similarity. Note: Associated with intracytoplasmic membranes By similarity.
Isoform BimEL: Mitochondrion
Isoform BimL: Mitochondrion
Isoform BimS: Mitochondrion
Isoform Bim-alpha1: Mitochondrion
Tissue specificity: Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. Ref.3
Domain: The BH3 motif is required for Bcl-2 binding and cytotoxicity.
Sequence similarities: Belongs to the Bcl-2 family.
Qty/Pk:
100 μg
Brand Family:
Chemicon
Format:
Purified
Host:
Rabbit
Product Name:
Anti-Bim, internal epitope, pan-Bim isoforms
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody