Ordering Information
- : AB1889
- : 100 µg
- : 1 mg/mL
Product Images
Western blot analysis of CCR5 in THP-1 whole cell lysate with anti-CCR5 polyclonal antibody (Catalog Number AB1889) at 1:1000 dilution.
Anti-C-C Chemokine Receptor 5 Antibody, N-terminus | AB1889
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-C-C Chemokine Receptor 5 Antibody, N-terminus | AB1889
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Specific for human C-C Chemokine Receptor 5 (CCR5) at the N-terminus.
Epitope:
N-terminus
Immunogen:
Synthetic peptide from the N-terminal domain of human CCR5.
Background Information:
HIV and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G-protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (1,2). Among them, CCR5 is a principle coreceptor for macrophage- and dual-tropic HIV-1 strains fusion of entry of human white blood cells (3,4). The amino-terminal domain and the extracellular loops of CCR5 serve as HIV binding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.
Species Reactivity:
Human
Application Notes:
Western blot: 1:1000 - 1:2000 (THP-1 whole cell lysate can be used as a positive control)
Optimal working dilutions must be determined by end user.
Optimal working dilutions must be determined by end user.
Presentation:
Purified immunoglobulin. 100 μg purified IgG in 100 μL of PBS containing 0.2% sodium azide.
Storage Conditions:
Store at 2-8°C for up to 12 months.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CCR5
- CCCKR5
- CMKBR5
- chemr13
- CD195
- CKR5
- CHEMR13
- CC-CKR-5
- CKR-5
- CCR-5
Alternate Names:
CCR-5; CD195
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Western Blotting
Entrez Gene Summary:
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of this gene was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. This gene is located at the chemokine receptor gene cluster region. Two transcript variants encoding the same protein have been found for this gene.
UniProt Summary:
FUNCTION: SwissProt: P51681 # Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ions level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
SIZE: 352 amino acids; 40524 Da
SUBUNIT: Interacts with PRAF2. Interacts with HIV-1 surface protein gp120.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Found in promyelocytic cells.
PTM: Sulfation contributes to the efficiency of HIV-1 entry. & O-glycosylated, but not N-glycosylated.
SIMILARITY: SwissProt: P51681 ## Belongs to the G-protein coupled receptor 1 family.
SIZE: 352 amino acids; 40524 Da
SUBUNIT: Interacts with PRAF2. Interacts with HIV-1 surface protein gp120.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Found in promyelocytic cells.
PTM: Sulfation contributes to the efficiency of HIV-1 entry. & O-glycosylated, but not N-glycosylated.
SIMILARITY: SwissProt: P51681 ## Belongs to the G-protein coupled receptor 1 family.
Brand Family:
Chemicon
Product Name:
Anti-C-C Chemokine Receptor 5 Antibody, N-terminus | AB1889
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 µg
Format:
Purified
Host:
Rabbit