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Western blot analysis of recombinant caspase-3 in the presence (Lane 1) and absence (Lane 2) of blocking peptide.
Anti-Caspase 3, large subunit & proform
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
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Entrez Gene Number:
Entrez Gene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 an
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Description:
Anti-Caspase 3, large subunit & proform
Background Information:
Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.
Alternate Names:
- apopain
- procaspase3
Specificity:
AB1899 detects procaspase-3 (32 kDa) and the cleaved p20 fragment in cells undergoing apoptosis.
Key Applications:
Western Blotting
Application Notes:
Western blot:
A 1:100-1:200 dilution of a previous lot was used in WB.
Optimal working dilutions must be determined by end user.
A 1:100-1:200 dilution of a previous lot was used in WB.
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Human
- Mouse
- Rat
Immunogen:
Synthetic peptide
Epitope:
Large subunit & proform
Molecular Weight:
45 kDa full length, 32-35 kDa partial intermediate and the cleaved p17/20 kDa fragment in cells undergoing apoptosis
Control:
Skin/Tumor, Embryo
Presentation:
Purified rabbit polyclonal liquid in PBS, pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Gene Symbol:
- CASP3
- CPP-32
- Yama
- CASP-3
- CPP32B
- SCA-1
- CPP32
- EC 3.4.22.56 [Contains: Caspase-3 subunit p17 Caspase-3 subunit p12].
Antibody Category:
- Apoptosis & Cancer
- Metabolism
Antibody Sub-Category:
- Caspases
- Enzymes & Biochemistry
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
APur
Concentration:
0.2 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Affinity Purified
Host:
Rabbit
Purification Method:
Affinity purified immunoglobulin