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注文に関する情報
- : AB19021
- : 50 µL
Anti-α-Catenin
| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| Ca, H, M | ELISA, IP, WB, IC, IH(P) | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-α-Catenin
Trade Name:
Chemicon (Millipore)
Immunogen:
Recombinant GST-fusion protein (amino terminal; 576 amino acids)
Background Information:
The catenins are polypeptides that bind to the conserved cytoplasmic tail of cadherins and are required for cadherin function (Gumbiner et al. 1993). a-catenin is a 102 kDa protein that is associated with the cadherin indirectly through its interaction with beta-catenin and gamma-catenin (Fouquet et al. 1992). beta-Catenin and its homolog plakoglobin associate directly with the cadherin (Butz et al. 1994). Thus, the cell-cell adherens junction is a structure composed of the transmembrane cadherin, which is associated directly with either beta-catenin or plakoglobin, which in turn assoctiates directly with alpha-catenin. alpha-catenin then mediates the interaction between the cadherin-catenin complex and the actin cytoskeleton (Nagafuchi et al. 1994; Shahidul et al. 1996). alpha-catenin has been characterized as a tumor suppressor, suggesting that it may have a regulatory function in regulation to its structural role (Rudiger 1998).
Species Reactivity:
- Canine
- Human
- Mouse
Application Notes:
Western blot
Immunocytochemistry
Immunohistochemistry on formalin-fixed paraffin-embedded tissues*: 1:250-1:500 *requires pressure cooker antigen retrieval method (Nagafuchi et al. 1994).
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Immunocytochemistry
Immunohistochemistry on formalin-fixed paraffin-embedded tissues*: 1:250-1:500 *requires pressure cooker antigen retrieval method (Nagafuchi et al. 1994).
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Presentation:
Serum. Liquid containing no preservatives.
Storage Conditions:
Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CTNNA1
- FLJ36832
- alpha-E-catenin
- CAP102
- alpha-catenin
- alphaE-catenin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunoprecipitation
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: P35221 # Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. May play a crucial role in cell differentiation.
SIZE: 906 amino acids; 100071 Da
SUBUNIT: Binds MLLT4 and F-actin (By similarity). Interacts directly with PSEN1 and CTNNB1 to form part of the PSEN1/cadherin/catenin adhesion complex. Interacts with ARHGAP21.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Cell junction; Cytoplasmic side. Note=Found at cell-cell boundaries and probably at cell- matrix boundaries.
TISSUE SPECIFICITY: Expressed ubiquitously in normal tissues.
PTM: Sumoylated.
DISEASE:SwissProt: P35221 # Abnormalities of alpha-catenin are involved in the process of cancer invasion and metastasis.
SIMILARITY: SwissProt: P35221 ## Belongs to the vinculin/alpha-catenin family.
SIZE: 906 amino acids; 100071 Da
SUBUNIT: Binds MLLT4 and F-actin (By similarity). Interacts directly with PSEN1 and CTNNB1 to form part of the PSEN1/cadherin/catenin adhesion complex. Interacts with ARHGAP21.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cell junction, adherens junction; Cytoplasmic side. Cell junction; Cytoplasmic side. Note=Found at cell-cell boundaries and probably at cell- matrix boundaries.
TISSUE SPECIFICITY: Expressed ubiquitously in normal tissues.
PTM: Sumoylated.
DISEASE:SwissProt: P35221 # Abnormalities of alpha-catenin are involved in the process of cancer invasion and metastasis.
SIMILARITY: SwissProt: P35221 ## Belongs to the vinculin/alpha-catenin family.
Qty/Pk:
50 µL
Brand Family:
Chemicon
Format:
Serum
Host:
Rabbit
Product Name:
Anti-α-Catenin
Antibody Type:
Polyclonal Antibody