Anti-beta-Catenin

P35222

FUNCTION: SwissProt: P35222 # Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
SIZE: 781 amino acids; 85497 Da
SUBUNIT: Two separate pools are found in the cytoplasm: one is PSEN1/cadherin/catenin complex which anchors to the actin cytoskeleton. The other pool is part of a large complex containing AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. Wnt- dependent activation of DVL antagonizes the action of GSK3B. When GSK3B activity is inhibited the complex dissociates, CTNNB1 is dephosphorylated and is no longer targeted for destruction. The stabilized protein translocates to the nucleus, where it binds TCF/LEF-1 family members, TBP, BCL9 and possibly also RUVBL1 and DUPLIN. Binds CTNNBIP and EP300. CTNNB1 forms a ternary complex with LEF1 and EP300 that is disrupted by CTNNBIP1 binding (By similarity). Interacts with the PDZ domain of TAX1BP3; this interaction inhibits the transcriptional activity of CTNNB1 (By similarity). Interacts with AJAP1, BAIAP1, CARM1, CTNNA3, CXADR and PCDH11Y. Binds SLC9A3R1. Interacts with GLIS2. Interacts with SLC30A9 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 promotes nuclear translocation.
TISSUE SPECIFICITY: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheats. Expressed in colon.
PTM: Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr- 41 to Ser-37 and Ser-33. & EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. & Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X (Probable). Its ubiquitination leads to its subsequent proteasomal degradation.
DISEASE: "SwissProt: P35222 # Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life. & Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor. & Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. & A chromosomal rearrangement involving CTNNB1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1."
SIMILARITY: SwissProt: P35222 ## Belongs to the beta-catenin family. & Contains 12 ARM repeats ... 隠す » もっと見る »

NM_001098209.1

Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM]. ... 隠す » もっと見る »

Anti-beta-Catenin

Catenins are cytosolic proteins found in varying abundance in many developing and adult tissues. Plakoglobin and beta-catenin (95 kDa) are homologous proteins and can subsitute for one another in the cadherin/catenin complex. Their activities are controlled by three types of interactions: those with cadherins in adherens junctions, linking them to the actin cytoskeleton; interactions in the nucleus, where they bind to transcription factors and stimulate gene expression; interactions of free cytoplasmic beta-catenin with axin and adenomatous polyposis coli (APC) protein which target it for degradation. Studies in the past year have demonstrated the complex interplay between these three types of interactions and the different behavior of beta-catenin and plakoglobin. Their involvement in morphogenesis and tumorigenesis strongly suggests that catenins play key roles in adhesion-mediated signaling.

Beta-catenin

Beta-catenin protein, antibody also cross reacts with mouse and dog. Beta-catenin is highly conserved between mammals and sequence comparisons show 98% similarity in pig and rat species as well

• ELISA
• Immunocytochemistry
• Immunohistochemistry
• Immunohistochemistry (Paraffin)
• Immunoprecipitation
• Western Blotting

Immunocytochemistry:
A previous lot of this antibody was used in immunocytochemistry.

Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.

Immunohistochemistry(paraffin):
A 1:250-1:500 dilution from a previous lot was used on formalin-fixed paraffin-embedded tissues*.
*Requires pressure cooker antigen retrieval method. Sections are baked overnight at 60°C, and then deparaffinized and treated for antigen retrieval by immersion in 6.6mM sodium citrate pH 6.0 for 5 minutes in a conventional pressure cooker. Sections were then blocked with 0.3% bovine serum albumin in Tris-buffered saline pH 8.0. Incubations are usually overnight, before washing 7 times with TBS plus 0.01% triton X-100 in the sixth wash.

ELISA:
A previous lot of this antibody was used in ELISA.
Western blot Analysis:1:500 dilution of this lot was used.
Optimal working dilutions must be determined by end user.

• Human
• Mouse
• Rat
• Canine

Recombinant GST-fusion protein (full length human beta-catenin)

94 kDa

MDBK cells, human lung tumour lysate

Rabbit polyclonal serum containing no preservatives.

Stable for 1 year at -20°C, maintain frozen, in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• CTNNB1
• FLJ25606
• CTNNB
• Beta-catenin
• PRO2286
• FLJ37923
• DKFZp686D02253

Routinely evaluated by Western Blot on L6 lysates.

Western blot Analysis: 1:500 dilution of this lot detected BETA CATENIN on 10 μg of L6 lysates.

Epigenetics & Nuclear Function

Transcription Factors

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Chemicon (Millipore)

Serum

Variable

Polyclonal Antibody

Serum

Rabbit

Unpurified.