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Ordering Information
- : AB19026
- : 100 µg
- : 1 mg/mL
Product Images
Western Blot Analysis:
RAW264.7 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-ADAM 10 (1:1000 dilutio...
Western Blot Analysis:
Representative image from a previous lot.
C2C12 lysate (Lane1), NIH/3T3 lysate (Lane 2) and RAW264.7 lysate (Lane 3) ...
Anti-ADAM 10, C-terminus
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-ADAM 10, C-terminus
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes ADAM 10, C-terminus.
Molecular Weight:
80-85 kDa
Epitope:
a.a. 732-748 of human ADAM 10
Immunogen:
Raised against a peptide corresponding to amino acids 732-748 of human ADAM 10 (Rosendahl, M.S., 1997). This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM 10 by one amino acid (Pan, D., 1997; Howard, L., 1996).
Background Information:
ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form. It is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity.
Tumor-necrosis factor alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNA-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10-, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloproteinase domain), was recently identified to cleave TNF-alpha, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10mRNA is expressed in a variety of human and bovine tissues.
Tumor-necrosis factor alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNA-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10-, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloproteinase domain), was recently identified to cleave TNF-alpha, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10mRNA is expressed in a variety of human and bovine tissues.
Species Reactivity:
- Bovine
- Human
- Mouse
- Rat
Species Reactivity Note:
Human, mouse and bovine.
Expected to react with rat based on sequence homology
Expected to react with rat based on sequence homology
Application Notes:
Western blot:
1:500 - 1:2,000 dilution. Jurkat whole cell lysate can be used as a positive control and an 85 kDa band can be detected which may represent precursor. A 60 kDa faint band was detected in some cell lines including Jurkat, which appears to be the processed mature protein. Overloading of the primary antibody can lead to multiple bands appearing in some preparations. The presence of glycosylated forms and breakdown products can lead to other bands. A prominent proform at near 80-85 is usually seen, with bands at 60, and below 50 kDa in most cases. The immunogen peptide is available (Cat. No. AG533) for performing antibody blocking studies.
Optimal working dilutions must be determined by end user.
1:500 - 1:2,000 dilution. Jurkat whole cell lysate can be used as a positive control and an 85 kDa band can be detected which may represent precursor. A 60 kDa faint band was detected in some cell lines including Jurkat, which appears to be the processed mature protein. Overloading of the primary antibody can lead to multiple bands appearing in some preparations. The presence of glycosylated forms and breakdown products can lead to other bands. A prominent proform at near 80-85 is usually seen, with bands at 60, and below 50 kDa in most cases. The immunogen peptide is available (Cat. No. AG533) for performing antibody blocking studies.
Optimal working dilutions must be determined by end user.
Control:
ADAM 10 is ubiquitously expressed. MDA-MB231 (Human breast cancer cell line known to express high levels of ADAM 10).
Quality Assurance:
Routinely evaluated by Western Blot on RAW264.7 lysates.
Western Blot: 1:500 dilution of this lot detected ADAM 10 on 10 μg of RAW264.7 lysates
Western Blot: 1:500 dilution of this lot detected ADAM 10 on 10 μg of RAW264.7 lysates
Purification Method:
Protein A purfied
Presentation:
Purified rabbit polyclonal IgG in buffer containing PBS and 0.02% sodium azide.
Storage Conditions:
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- AD10
- CD156c
- CDw156
- EC 3.4.24.81
- HsT18717
- KUZ
- MADM
- kuz
- kuzbanian
Alternate Names:
- A disintegrin and metalloproteinase domain 10
- ADAM metallopeptidase domain 10
- CD156c antigen
- Kuzbanian protein homolog
- Mammalian disintegrin-metalloprotease
- a disintegrin and metalloprotease domain 10
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin.
Key Applications:
Western Blotting
UniProt Summary:
FUNCTION: SwissProt: O14672 # Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala- -Val-77' to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (By similarity).
COFACTOR: Binds 1 zinc ion (By similarity).
SIZE: 748 amino acids; 84142 Da
SUBUNIT: Interacts with ephrin-A2 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Intracytoplasmic membrane; Single-pass type I membrane protein. Note=Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
TISSUE SPECIFICITY: Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
DOMAIN: SwissProt: O14672 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase (By similarity).
SIMILARITY: Contains 1 disintegrin domain. & Contains 1 peptidase M12B domain.
COFACTOR: Binds 1 zinc ion (By similarity).
SIZE: 748 amino acids; 84142 Da
SUBUNIT: Interacts with ephrin-A2 (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Intracytoplasmic membrane; Single-pass type I membrane protein. Note=Is localized in the plasma membrane but is predominantly expressed in the Golgi apparatus and in released membrane vesicles derived likely from the Golgi.
TISSUE SPECIFICITY: Expressed in spleen, lymph node, thymus, peripheral blood leukocyte, bone marrow, cartilage, chondrocytes and fetal liver.
DOMAIN: SwissProt: O14672 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase (By similarity).
SIMILARITY: Contains 1 disintegrin domain. & Contains 1 peptidase M12B domain.
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Purified
Host:
Rabbit
Product Name:
Anti-ADAM 10, C-terminus
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody