Product Images
Rabbit anti-Neurofilament 145 (Catalog Number AB1987) staining of rat enteric neurons.
Immunohistochemistry(paraffin): Representative image from a previous lot.
Neurofilament-M (cat. #: AB1987) staining on Normal Cerebellum. Tissue...
 staining of rat enteric neurons.'))
: Representative image from a previous lot.<br />Neurofilament-M (cat. #: AB1987) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. The antibody was diluted to 1:200, using IHC-Select® Detection with HRP-DAB.'))
: Representative image from a previous lot.<br />Negative Control staining of Cerebellum. Tissue pretreated with Citrate Buffer, pH 6.0. Negative Control Reagent, using IHC-Select® Detection with HRP-DAB. No background staining i'))
. <br />Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated'))
Anti-Neurofilament M (145 kDa) Antibody, C-terminus | AB1987
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, Po, R, Qu, Rb, Rp | ICC, IHC, IH(P), WB | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Neurofilament M (145 kDa) Antibody, C-terminus | AB1987
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Strong and specific staining for NF-M (145 kDa)
Molecular Weight:
145 kDa
Epitope:
C-terminus
Immunogen:
Recombinant fusion protein containing the C-terminal 168 amino acids of rat NF-M
Background Information:
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
Species Reactivity:
- Bovine
- Chicken
- Human
- Mouse
- Pig
- Rat
- Quail
- Rabbit
- Reptile
Species Reactivity Note:
Rat, human, mouse, pig, cow(bovine), chicken, quail, alligator and tortoise.
Application Notes:
Immunohistochemistry:
A 1:200 dilution of a previous lot was used in Immunohistochemistry.
Staining is not affected by neurofilament phosphorylation. Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
Immunohistochemistry(paraffin):
Optimal working dilutions must be determined by end user.
A 1:200 dilution of a previous lot was used in Immunohistochemistry.
Staining is not affected by neurofilament phosphorylation. Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
Immunohistochemistry(paraffin):
Optimal working dilutions must be determined by end user.
Control:
Rat enteric neurons, rat spinal cord tissue Brain, Peripheral Nerve
Quality Assurance:
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot: 1:1000 dilution of this lot detected Neurofilament M on 10 μg of mouse brain lysates..
Western Blot: 1:1000 dilution of this lot detected Neurofilament M on 10 μg of mouse brain lysates..
Purification Method:
Unpurified
Presentation:
Neat rabbit antiserum containing no preservative.
Storage Conditions:
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- NEFM
- NF-M
- NEF3
- NFM
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P07197 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
SIZE: 916 amino acids; 102448 Da
PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family.
SIZE: 916 amino acids; 102448 Da
PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family.
Brand Family:
Chemicon
Product Name:
Anti-Neurofilament M (145 kDa) Antibody, C-terminus | AB1987
Concentration:
Variable
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 µL
Format:
Serum
Host:
Rabbit