Ordering Information
- : AB3209
- : 100 μg
Anti-Oct-4 Antibody | AB3209
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | ICC, IHC, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Oct-4 Antibody | AB3209
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Oct4 (POU5F1). The molecular weight of the protein is 38,601 Daltons.
Molecular Weight:
44 kDa
Immunogen:
Synthetic peptide, amino acids 282-297of Oct4.
Background Information:
Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
Species Reactivity:
- Human
- Mouse
Application Notes:
Western blot: 1:200-1:1,000 using ECL. Oct-4 in cells typically shows ~40-44kDa band or doublet.
Immunohistochemistry: 1:200-1:1,000.
Immunocytochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:200-1:1,000.
Immunocytochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.
Control:
Pluripotent human embryonic stem (ES) cells
Purification Method:
Immunoaffinity purified
Presentation:
Affinity Purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Storage Conditions:
Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- POU5F1
- Oct-3
- OTF4
- OCT3
- OCT4
- Oct-4
- MGC22487
- Oct4
- OTF3
Alternate Names:
POU5F1
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: Q01860 # Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Prime candidate for an early developmental control gene.
SIZE: 360 amino acids; 38571 Da
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
SIZE: 360 amino acids; 38571 Da
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Ubiquitous. Expressed at low levels.
SIMILARITY: SwissProt: Q01860 ## Belongs to the POU transcription factor family. Class- 5 subfamily. & Contains 1 homeobox DNA-binding domain. & Contains 1 POU-specific domain.
MISCELLANEOUS: Several pseudogenes of POU5F1 have been described on chromosomes 1, 3, 8, 10 and 12. 2 of them, localized in chromosomes 8 and 10, are transcribed in cancer tissues but not in normal ones and may be involved in the regulation of POU5F1 gene activity in carcinogenesis.
Brand Family:
Chemicon
Product Name:
Anti-Oct-4 Antibody | AB3209
Antibody Type:
Polyclonal Antibody
Qty/Pk:
100 μg
Format:
Affinity Purified
Host:
Rabbit