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Lane 1: Untreated non-apoptotic HeLa cell extract . Lane 2: HeLa cells treated with Camptothecin (apoptotic).
Rabbit anti-Caspase 3, Active (Catalog Number AB3623). Localization of Active Caspase (red) in trimethyltin (TMT) treated mouse brain (dentate gyrus (...
Anti-Caspase 3, active (cleaved) form
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IF, IH, IH(P), WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Caspase 3, active (cleaved) form
Trade Name:
Chemicon (Millipore)
Specificity:
AB3623 detects only the cleaved p17 fragment and does not detect the precursor form in cells undergoing apoptosis.
Molecular Weight:
17 kDa
Epitope:
active (cleaved) form
Immunogen:
Cleavage specific synthetic peptide sequence (proprietary).
Background Information:
Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human, Mouse, Rat. Reactivity with other species has not been determined.
Application Notes:
Western Blot Analysis:
1:100-1:200 (0.5-4 µg/mL) dilution of active apoptotic cells we used to detect Caspase-3.
Note when using tissue, levels of active caspase 3 can below detectable levels. Use CC119 (human caspase 3) incubated 30 minutes with 1 mM ATP (for activation) as a positive control on westerns. Alternatively, neuronal culture preparations incubated with staurosporine (0.5 µM, 10-18 hr) typically give a strong caspase 3 signal.
Immunohistochemistry:
1:10 dilution (10-20 µg/mL), AB3623 has reacted successfully in 4% PFA fixed, paraffin embedded tissues mouse tissues.
Immunohistochemistry(paraffin):
Optimal Staining of Caspase-3 Monoclonal Antibody: Mouse Small Intestine
1:100-1:200 (0.5-4 µg/mL) dilution of active apoptotic cells we used to detect Caspase-3.
Note when using tissue, levels of active caspase 3 can below detectable levels. Use CC119 (human caspase 3) incubated 30 minutes with 1 mM ATP (for activation) as a positive control on westerns. Alternatively, neuronal culture preparations incubated with staurosporine (0.5 µM, 10-18 hr) typically give a strong caspase 3 signal.
Immunohistochemistry:
1:10 dilution (10-20 µg/mL), AB3623 has reacted successfully in 4% PFA fixed, paraffin embedded tissues mouse tissues.
Immunohistochemistry(paraffin):
Optimal Staining of Caspase-3 Monoclonal Antibody: Mouse Small Intestine
Control:
Tonsil and appendix tissue. Jurkat cells induced for apoptosis with recombinant soluble TRAIL and staurosporine treated mouse 3T3 cell lysates Skin/Tumor, Embryo.
Purification Method:
Immunoaffinity purified
Presentation:
Purified rabbit polyclonal in buffer containing PBS, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CASP3
- CPP-32
- Yama
- CASP-3
- CPP32B
- SCA-1
- CPP32
- EC 3.4.22.56 [Contains: Caspase-3 subunit p17 Caspase-3 subunit p12].
Alternate Names:
- Apopain
- Yama Protein
- CASP3
- SCA-1
- procaspase3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunofluorescence
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Entrez Gene Summary:
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
UniProt Summary:
FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
SIZE: 277 amino acids; 31608 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
SIZE: 277 amino acids; 31608 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.
Brand Family:
Chemicon
Product Name:
Anti-Caspase 3, active (cleaved) form
Concentration:
0.2 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 μg
Format:
Affinity Purified
Host:
Rabbit