Ordering Information
Product Images
Lane 1: Untreated non-apoptotic HeLa cell extract . Lane 2: HeLa cells treated with Camptothecin (apoptotic).
Rabbit anti-Caspase 3, Active (Catalog Number AB3623). Localization of Active Caspase (red) in trimethyltin (TMT) treated mouse brain (dentate gyrus (...
Anti-Caspase 3, active (cleaved) form
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IF, IH, IH(P), WB | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
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Entrez Gene Number:
Entrez Gene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 an
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Description:
Anti-Caspase 3, active (cleaved) form
Background Information:
Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.
Alternate Names:
- Apopain
- Yama Protein
- CASP3
- SCA-1
- procaspase3
Specificity:
AB3623 detects only the cleaved p17 fragment and does not detect the precursor form in cells undergoing apoptosis.
Key Applications:
- Immunofluorescence
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Application Notes:
Western Blot Analysis:
1:100-1:200 (0.5-4 µg/mL) dilution of active apoptotic cells we used to detect Caspase-3.
Note when using tissue, levels of active caspase 3 can below detectable levels. Use CC119 (human caspase 3) incubated 30 minutes with 1 mM ATP (for activation) as a positive control on westerns. Alternatively, neuronal culture preparations incubated with staurosporine (0.5 µM, 10-18 hr) typically give a strong caspase 3 signal.
Immunohistochemistry:
1:10 dilution (10-20 µg/mL), AB3623 has reacted successfully in 4% PFA fixed, paraffin embedded tissues mouse tissues.
Immunohistochemistry(paraffin):
Optimal Staining of Caspase-3 Monoclonal Antibody: Mouse Small Intestine
1:100-1:200 (0.5-4 µg/mL) dilution of active apoptotic cells we used to detect Caspase-3.
Note when using tissue, levels of active caspase 3 can below detectable levels. Use CC119 (human caspase 3) incubated 30 minutes with 1 mM ATP (for activation) as a positive control on westerns. Alternatively, neuronal culture preparations incubated with staurosporine (0.5 µM, 10-18 hr) typically give a strong caspase 3 signal.
Immunohistochemistry:
1:10 dilution (10-20 µg/mL), AB3623 has reacted successfully in 4% PFA fixed, paraffin embedded tissues mouse tissues.
Immunohistochemistry(paraffin):
Optimal Staining of Caspase-3 Monoclonal Antibody: Mouse Small Intestine
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human, Mouse, Rat. Reactivity with other species has not been determined.
Immunogen:
Cleavage specific synthetic peptide sequence (proprietary).
Epitope:
active (cleaved) form
Molecular Weight:
17 kDa
Control:
Tonsil and appendix tissue. Jurkat cells induced for apoptosis with recombinant soluble TRAIL and staurosporine treated mouse 3T3 cell lysates Skin/Tumor, Embryo.
Presentation:
Purified rabbit polyclonal in buffer containing PBS, containing 50% glycerol, 1% BSA and 0.02% thimerosal.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Gene Symbol:
- CASP3
- CPP-32
- Yama
- CASP-3
- CPP32B
- SCA-1
- CPP32
- EC 3.4.22.56 [Contains: Caspase-3 subunit p17 Caspase-3 subunit p12].
Antibody Category:
- Apoptosis & Cancer
- Metabolism
Antibody Sub-Category:
- Caspases
- Enzymes & Biochemistry
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Pur
Concentration:
0.2 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Affinity Purified
Host:
Rabbit
Purification Method:
Immunoaffinity Purified