Anti-CXC Chemokine Receptor 3 Antibody, C-terminal domain | AB4250
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IH(P) | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-CXC Chemokine Receptor 3 Antibody, C-terminal domain | AB4250
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes human CXCR3. The specificity of this antibody has been evaluated by IHC staining of human tonsil.
Epitope:
C-terminal domain
Immunogen:
Synthetic peptide corresponding to a sequence within the C-terminal domain of human CXCR3. The immunogen has 82% homology with rat and mouse.
Background Information:
CXCR3, a chemokine receptor, is highly similar to the interleukin 8B receptor and has been shown to mediate recruitment of T cells to sites of inflammation and retention of T-cells in the inflamed CNS. CXCR3 has been suggested to be the principal inflammatory chemokine receptor involved in intrathecal accumulation of T-cells in multiple sclerosis and diabetes. It is expressed on malignant B cells in chronic lymphoproliferative disorders (Jones et al., 2000). An alternatively spliced isoform, CXCR3B, has recently been identified (Lasagni et al., 2003). CXCR3 expression has been documented in the brain, lymphoid and other peripheral tissues, and in diseases including atherosclerosis, Alzheimer's, multiple sclerosis, rheumatoid arthritis, lymphomas, asthma, neoplasms, leukemia, and inflammatory conditions of various organs.
Species Reactivity:
Human
Application Notes:
Immunohistochemistry (formalin-fixed paraffin-embedded tissues)
Optimal working dilutions must be determined by the end user.
Optimal working dilutions must be determined by the end user.
Presentation:
Peptide-affinity purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.7, containing 0.01% sodium azide
Storage Conditions:
Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. May be stored at 2-8°C for short term use. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- CXCR3
- CD182
- CXC-R3
- IP10-R
- IP10
- Mig-R
- CD183
- CKR-L2
- CMKAR3
- GPR9
- CXCR-3
- MigR
Alternate Names:
CXCR3
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
Immunohistochemistry (Paraffin)
Entrez Gene Summary:
CD183 is a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Historically, CD183 is the third CXC chemokine receptor discovered and, therefore, commonly designated as CXCR3. Binding of chemokines to CD183 induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to CD183. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. A hallmark of CD183 is its prominent expression in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that CD183 and its chemokines participate in the recruitment of inflammatory cells. Therefore, CD183 is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases.
UniProt Summary:
FUNCTION: SwissProt: P49682 # Receptor for CXCL9, CXCL10 and CXCL11 and mediates the proliferation of human mesangial cells (HMC). Isoform 2 is a receptor for CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the growth of human microvascular endothelial cells (HMVEC). Isoform 2 may play a role in angiogenesis. Isoform 3 mediates activity of CXCL11.
SIZE: 368 amino acids; 40660 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
SIMILARITY: SwissProt: P49682 ## Belongs to the G-protein coupled receptor 1 family.
SIZE: 368 amino acids; 40660 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta.
SIMILARITY: SwissProt: P49682 ## Belongs to the G-protein coupled receptor 1 family.
Brand Family:
Chemicon
Product Name:
Anti-CXC Chemokine Receptor 3 Antibody, C-terminal domain | AB4250
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Qty/Pk:
50 µg
Format:
Affinity Purified
Host:
Rabbit