Informações para Pedidos
Product Images
Rabbit anti-NG2 (Catalog Number AB5320) staining of adult rat cortex. Photo shows oligodendrocyte precursor cell.
Western Blot Analysis:
Rat brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-NG2 Chondroitin Sulfate...
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Anti-NG2 Chondroitin Sulfate Proteoglycan
| Reatividade à Espécie | Aplicações Essenciais | Hospedeiro | Formato | Tipo de Anticorpo |
|---|---|---|---|---|
| H, M, R, Mk | ELISA, IC, IH, IP, WB | Rabbit | Affinity Purified | Polyclonal Antibody |
Número Uniprot:
UniProt Summary
FUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate M
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Número Genbank:
Entrez Gene Summary
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
Description:
Anti-NG2 Chondroitin Sulfate Proteoglycan
Background Information:
NG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.
Alternate Names:
- Chondroitin sulfate proteoglycan NG2
- Melanoma chondroitin sulfate proteoglycan 3
- Melanoma-associated chondroitin sulfate proteoglycan
- chondroitin sulfate proteoglycan 4
- chondroitin sulfate proteoglycan 4 (melanoma-associated)
Specificity:
NG2 Chondroitin Sulfate Proteoglycan. AB5320 identifies both the intact proteoglycan and the core protein by Western blot and ELISA. When oligodendrocyte precursor cells (i.e. O-2A progenitor cells) are stained alive, the stain appears as clusters on the cell surface. This antibody does not stain differentiated oligodendrocytes well.
Key Applications:
- ELISA
- Immunocytochemistry
- Immunohistochemistry
- Immunoprecipitation
- Western Blotting
Application Notes:
Immunocytochemistry:
A 1:150-1:600 dilution of a previous lot was used in IC.
Immunohistochemistry:
1:200 dilution of a previous lot was used on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.
Immunoprecipitation:
2 μg/mL concentration of a previous lot was used in IP.
ELISA:
A 1:1,500-1:3,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions and protocols must be determined by end user
A 1:150-1:600 dilution of a previous lot was used in IC.
Immunohistochemistry:
1:200 dilution of a previous lot was used on frozen sections of embryonic mouse brain using an Alexa Fluor conjugated secondary antibody. Care should be taken to avoid overfixing tissue sections. A short (5-10 minute) fix in 2-4% PFA is recommended.
Immunoprecipitation:
2 μg/mL concentration of a previous lot was used in IP.
ELISA:
A 1:1,500-1:3,000 dilution of a previous lot was used in ELISA.
Optimal working dilutions and protocols must be determined by end user
Species Reactivity:
- Human
- Mouse
- Rat
- Monkey
Immunogen:
Immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat.
Molecular Weight:
270-300 kDa
Control:
Human melanoma, glioma and proliferating brain endothelial cells, rat B49 glial cell extracts or embryonic mouse brain tissue.
Presentation:
Purified rabbit polyclonal in PBS buffer containing 0.02% azide.
Storage Conditions:
Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Gene Symbol:
- CSPG4
- MSK16
- MCSP
- NG2
- MCSPG
- MEL-CSPG
- HMW-MAA
Quality Assurance:
Routinely evaluated by Western Blot on rat brain lysates
Western Blot: 1:1000 dilution of this lot detected NG2 chondroitin sulfate on 10 μg of rat brain lysates.
Western Blot: 1:1000 dilution of this lot detected NG2 chondroitin sulfate on 10 μg of rat brain lysates.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format Code:
Pur
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Affinity Purified
Host:
Rabbit
Purification Method:
Antibody purified by protein A, then placed over a fibronectin affinity column.