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Rabbit anti-alpha-internexin (Catalog Number AB5354) Staining of adult rat sensory neurons (40X).
Rabbit anti-alpha-internexin (Catalog Number AB5354) and Mouse anti-NF-L (Catalog Number MAB1615) staining of cultured rat neurons. Mature neurons are...
Anti-Internexin Antibody, α | AB5354
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Ch, Ma | ELISA, WB, ICC, IHC | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Internexin Antibody, α | AB5354
Promotional Text:
Trade Name:
Chemicon (Millipore)
Specificity:
alpha-internexin, C-terminal globular "tail" region. By western blot, AB5354 detects a major band at 66 kDa and a minor band at approximately 150 kDa corresponding to alpha internexin monomer and dimer, respectively. A minor band at approximately 50 kDa is occasionally detected and is believed to be a degradation product of alpha internexin. This antibody has been referred to in the literature as the R36 antibody (Evans, 2002).
Immunogen:
Full length recombinant rat alpha-internexin fused to E. coli Trp E.
Species Reactivity:
- Chicken
- Mammals
Application Notes:
Immunohistochemistry on formalin or paraformaldehyde fixed tissues.
Immunocytochemistry.
Western blot: using human or rat brain homogenates.
ELISA
Optimal working dilutions must be determined by end user.
Immunocytochemistry.
Western blot: using human or rat brain homogenates.
ELISA
Optimal working dilutions must be determined by end user.
Control:
POSITIVE CONTROL CELLS: PC12, Ntera2.
Presentation:
Serum liquid, containing no preservatives.
Storage Conditions:
Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- INA
- NF-66
- NEF5
- MGC12702
- Alpha-Inx
- TXBP-1
- Neurofilament-66
- Alpha-internexin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: Q16352 # Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
SIZE: 499 amino acids; 55391 Da
TISSUE SPECIFICITY: Found predominantly in adult CNS.
DEVELOPMENTAL STAGE: Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
PTM: O-glycosylated (By similarity). & Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: Q16352 ## Belongs to the intermediate filament family.
SIZE: 499 amino acids; 55391 Da
TISSUE SPECIFICITY: Found predominantly in adult CNS.
DEVELOPMENTAL STAGE: Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
PTM: O-glycosylated (By similarity). & Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: SwissProt: Q16352 ## Belongs to the intermediate filament family.
Qty/Pk:
50 µL
Brand Family:
Chemicon
Format:
Serum
Host:
Rabbit
Product Name:
Anti-Internexin Antibody, α | AB5354
Antibody Type:
Polyclonal Antibody