Anti-Capsaicin Receptor, C-terminus

Anti-Capsaicin Receptor, C-terminus

Chemicon (Millipore)

Capsaicin Receptor (Vanilloid Receptor 1, VR1).

C-terminus

A 21 amino acid peptide corresponding to the C-terminus of the rat Capsaicin Receptor protein.

• Human
• Rat

Immunohistochemistry: 1-2 μg/mL.

The antibody reacts well with tissue from the rat dorsal root ganglia.

Optimal working dilutions must be determined by end user.

Affinity purified immunoglobulin. Lyophilized. Contains no preservative. Reconstitute with 20 μL of sterile distilled water. Centrifuge if necessary to remove any residue.

Maintain lyophilized material at -20°C to -70°C for up to 6 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.

Q8NER1

• NM_018727.5
• NM_080704.3
• NM_080705.3
• NM_080706.3

• TRPV1
• VR1
• OTRPC1
• DKFZp434K0220
• TrpV1

Vanilloid Receptor 1; VR1

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene.

Immunohistochemistry

FUNCTION: SwissProt: Q8NER1 # Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL.
SIZE: 839 amino acids; 94938 Da
SUBUNIT: Self-associates. Probably homotetramer. May also form a heteromeric channel with TRPV3. Interacts with calmodulin, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity).
TISSUE SPECIFICITY: Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).DOMAIN:SwissProt: Q8NER1 The association domain (AD) is necessary for self- association (By similarity).
PTM: Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity (By similarity).
SIMILARITY: Belongs to the transient receptor family. TrpV subfamily. & Contains 3 ANK repeats.
MISCELLANEOUS: Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine.

20 µg

Chemicon

Affinity Purified

Rabbit

Anti-Capsaicin Receptor, C-terminus

Polyclonal Antibody