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Goat anti-RAGE (Catalog Number AB5484) staining of diabetic blood vessels.
Anti-RAGE
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, IH(P) | Goat | Serum | Polyclonal Antibody |
Description:
Anti-RAGE
Trade Name:
Chemicon (Millipore)
Specificity:
Specific for receptor for advanced glycosylation end products (RAGE). This antibody immunolabels vessels from diabetic tissues and vessels and neurons from the brains of Alzheimer's Disease (AD) patients in formalin-fixed paraffin sections.
Immunogen:
Synthetic peptide that corresponds to amino acids 42-59 of the human receptor for advanced glycated end products.
Background Information:
RAGE is a member of the immunoglobulin superfamily of cell surface molecules that binds molecules that have been irreversibly modified by non-enzymatic glycation and oxidation, and are know as advanced glycation end products (AGEs). It is expressed by endothelium, mononuclear phagocytes, neurons and smooth muscle cells. Whereas RAGE is present at high levels during development, especially in the central nervous system, its levels decline during maturity.The increased expression of RAGE is associated with several pathological states, such as diabetic vasculopathy, neuropathy, retinopathy and other disorders, including Alzheimer's disease and immune/inflammatory reactions of the vessel walls. In diabetic tissues, the production of RAGE is due to the overproduction of AGEs that eventually overwhelm the protective properties of RAGE. This results in oxidative stress and endothelial cell dysfunction that leads to vascular disease in diabetics. In the brain, RAGE also binds amyloid beta (Ab). Because Ab is overproduced in neurons and vessels in the brains of Alzheimer disease, this leads to the hyperstimulation of RAGE. The RAGE-Ab interaction is thought to result in oxidative stress leading to neuronal degeneration.
Species Reactivity:
Human
Application Notes:
Immunohistochemistry on formalin fixed, paraffin embedded tissue sections: = 1:400. The antibody reacts well with human diabetic kidney. The antibody may show better reactivity after performing antigen retrieval by the citrate buffer/pressure cooker method.
ELISA against immunogen peptide: = 1:260,000
Optimal working dilutions must be determined by the end user.
ELISA against immunogen peptide: = 1:260,000
Optimal working dilutions must be determined by the end user.
Presentation:
Goat serum. Liquid containing 0.01% thimerosal.
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- RAGE
- RAGE-1
- MOK
- RAGE1
- EC 2.7.11.22
Alternate Names:
Receptor for Advanced Glycosylation End Products
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- ELISA
- Immunohistochemistry (Paraffin)
UniProt Summary:
FUNCTION: SwissProt: Q9UQ07 # Able to phosphorylate several exogenous substrates and to undergo autophosphorylation (By similarity).
SIZE: 419 amino acids; 48014 Da
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
TISSUE SPECIFICITY: Expressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina.
PTM: Autophosphorylated (By similarity).
SIMILARITY: SwissProt: Q9UQ07 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
SIZE: 419 amino acids; 48014 Da
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
TISSUE SPECIFICITY: Expressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina.
PTM: Autophosphorylated (By similarity).
SIMILARITY: SwissProt: Q9UQ07 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Qty/Pk:
200 µL
Brand Family:
Chemicon
Format:
Serum
Host:
Goat
Product Name:
Anti-RAGE
Antibody Type:
Polyclonal Antibody