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注文に関する情報
- : AB5490
- : 100 μg
- : 1 mg/mL
Anti-Atrial Natriuretic Peptide
| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| M, R | IH, IP, RIA | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-Atrial Natriuretic Peptide
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes Atrial Natriuretic Peptide (ANP).
Immunogen:
Rat ANP conjugated to BSA.
Species Reactivity:
- Mouse
- Rat
Application Notes:
Immunohistochemistry: 2-10 μg/mL. See suggested protocol below.
Immunoprecipitation
RIA
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL. Anesthetize animals and perfuse them transcardially as follows: a) Flush with cold (4°C) oxygenated Calcium free Tyrodes b) Perfuse with 4% formaldehyde in 0.16M phosphate buffer at pH 6.9 c) Perfuse with 10% sucrose solution in 0.1M phosphate buffer (pH 7.2)Sections Cut 5-30 mm sections using a cryostat and mount them on subbed histological slides.ImmunofluorescenceDilute ANP antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Triton X-100. Incubate sections for 24-48 hours at 2-8°C. Wash in PBS (three times for 10 minutes). Incubate for one hour or two hours at room temperature with properly diluted donkey anti-rabbit secondary antibody conjugated to a fluorescent probe such as FITC (Catalog Number AP182F), Rhodamine (Catalog Number AB182R) or Cy3 (Catalog Number AP182C). Wash in PBS (three times for 10 minutes) and mount with a PBS/glycerol solution containing 0.1% phenylenediamine to reduce fading. If not stained with FITC, sections can be dehydrated in grading alcohols (50%, 75%, 80%, 96% and 100%), cleared with xylene and mounted with DPX (Fluka, Ronkonkoma, NY). Staining can be visualized by using both conventional and confocal microscopy.Indirect Avidin-Biotin PeroxidaseIncubate sections with 0.3% H2O2 in PBS for 15 minutes at room temperature to block endogenous peroxidase. Rinse sections with PBS (three times for 10 minutes). Dilute ANP antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Triton X-100. Incubate sections overnight at 2-8°C and then wash in PBS (three times for 10 minutes). Incubate sections with properly diluted biotinilated goat anti-rabbit secondary antibody (Catalog Number AP132B) diluted in PBS (do not add sodium azide!) for one hour at room temperature. Rinse sections three times for 15 minutes and incubate sections with horseradish peroxidase-streptavidin complex (Catalog Number SA108) properly diluted in PBS/0.1% Triton X-100 for 40 minutes at room temperature. Wash sections PBS and incubate them in substrate solutions (e.g. DAB, AEC, etc.) to achieve necessary intensity of ANP staining.
Immunoprecipitation
RIA
Optimal working dilutions must be determined by the end user.
IMMUNOHISTOCHEMISTRY PROTOCOL. Anesthetize animals and perfuse them transcardially as follows: a) Flush with cold (4°C) oxygenated Calcium free Tyrodes b) Perfuse with 4% formaldehyde in 0.16M phosphate buffer at pH 6.9 c) Perfuse with 10% sucrose solution in 0.1M phosphate buffer (pH 7.2)Sections Cut 5-30 mm sections using a cryostat and mount them on subbed histological slides.ImmunofluorescenceDilute ANP antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Triton X-100. Incubate sections for 24-48 hours at 2-8°C. Wash in PBS (three times for 10 minutes). Incubate for one hour or two hours at room temperature with properly diluted donkey anti-rabbit secondary antibody conjugated to a fluorescent probe such as FITC (Catalog Number AP182F), Rhodamine (Catalog Number AB182R) or Cy3 (Catalog Number AP182C). Wash in PBS (three times for 10 minutes) and mount with a PBS/glycerol solution containing 0.1% phenylenediamine to reduce fading. If not stained with FITC, sections can be dehydrated in grading alcohols (50%, 75%, 80%, 96% and 100%), cleared with xylene and mounted with DPX (Fluka, Ronkonkoma, NY). Staining can be visualized by using both conventional and confocal microscopy.Indirect Avidin-Biotin PeroxidaseIncubate sections with 0.3% H2O2 in PBS for 15 minutes at room temperature to block endogenous peroxidase. Rinse sections with PBS (three times for 10 minutes). Dilute ANP antibody with 0.1M phosphate buffered saline (PBS, pH 7.4) containing 1% BSA and 0.1% Triton X-100. Incubate sections overnight at 2-8°C and then wash in PBS (three times for 10 minutes). Incubate sections with properly diluted biotinilated goat anti-rabbit secondary antibody (Catalog Number AP132B) diluted in PBS (do not add sodium azide!) for one hour at room temperature. Rinse sections three times for 15 minutes and incubate sections with horseradish peroxidase-streptavidin complex (Catalog Number SA108) properly diluted in PBS/0.1% Triton X-100 for 40 minutes at room temperature. Wash sections PBS and incubate them in substrate solutions (e.g. DAB, AEC, etc.) to achieve necessary intensity of ANP staining.
Control:
Heart tissue
Purification Method:
Immunoaffinity purified
Presentation:
Affinity Purified immunoglobulin. Liquid containing 0.1% sodium azide.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- NPPA
- CDD-ANF
- ANP
- PND
- ANF
- pronatriodilatin
- OTTHUMP00000044485
- Prepronatriodilatin
Alternate Names:
ANP
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Immunohistochemistry
- Immunoprecipitation
- Radioimmunoassay
UniProt Summary:
FUNCTION: SwissProt: P01160 # A potent vasoactive substance which is thought to play a key role in cardiovascular homeostasis. Has a cGMP-stimulating activity.
SIZE: 153 amino acids; 16708 Da
SUBUNIT: Interacts with NPR3.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01160 ## Belongs to the natriuretic peptide family.
SIZE: 153 amino acids; 16708 Da
SUBUNIT: Interacts with NPR3.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01160 ## Belongs to the natriuretic peptide family.
Qty/Pk:
100 μg
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-Atrial Natriuretic Peptide
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody