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注文に関する情報
- : AB5585
- : 100 µL
- : Variable
画像情報
Rabbit anti-Recoverin (Catalog Number AB5585). Immunolocalization of recoverin in human retina.
Anti-Recoverin
当該製品は右記製品の推奨代替品です: AB5431P| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| H, M, R, Ch, Fg | IC, IH, IH(P), WB | Rabbit | Serum | Polyclonal Antibody |
Description:
Anti-Recoverin
Replaces:
AB5431P
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes recoverin.
Molecular Weight:
26 kDa
Immunogen:
Recombinant human recoverin.
Background Information:
Recoverin is a calcium-binding protein expressed in retinal photoreceptors and midget cone bipolar cells. It appears to delay the termination of the phototransduction cascade by blocking the phosphorylation of photoexcited rhodopsin.
Species Reactivity:
- Human
- Mouse
- Rat
- Chicken
- Frog
Species Reactivity Note:
Human, rat, mouse, chicken and frog. Other species have not been tested.
Application Notes:
Western Blot Analysis:
1:1,000-1:5,000 dilution using ECL.
Immunohistochemistry:
1:1,000 (2 hours at room temperature or overnight at 2-8ºC) on frozen and paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde or 3% paraformaldehyde with 0.1% glutaraldehyde. Suggested permeabilization solution is 0.1% Triton X-100. Suggested blocking buffer is 0.05 M Tris saline, pH 7.4 containing 2.5% BSA, 0.1% triton and 0.05% sodium azide.
Optimal working dilutions must be determined by the end user.
1:1,000-1:5,000 dilution using ECL.
Immunohistochemistry:
1:1,000 (2 hours at room temperature or overnight at 2-8ºC) on frozen and paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde or 3% paraformaldehyde with 0.1% glutaraldehyde. Suggested permeabilization solution is 0.1% Triton X-100. Suggested blocking buffer is 0.05 M Tris saline, pH 7.4 containing 2.5% BSA, 0.1% triton and 0.05% sodium azide.
Optimal working dilutions must be determined by the end user.
Control:
POSITIVE CONTROL: Recoverin is found in photoreceptors and midget cone bipolar cells in retina and pinealocytes in pineal gland.
Purification Method:
Unpurified
Presentation:
Rabbit polyclonal serum. Contains no preservative.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- RCVRN
- Recoverin
- RCV1
Alternate Names:
Recoverin
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
UniProt Summary:
FUNCTION: SwissProt: P35243 # Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
SIZE: 200 amino acids; 23130 Da
TISSUE SPECIFICITY: Retina and pineal gland.
DISEASE: SwissProt: P35243 # Identified as the antigen in cancer-associated retinopathy, an autoimmune disease of the retina caused by a tumor in another tissue.
SIMILARITY: SwissProt: P35243 ## Belongs to the recoverin family. & Contains 4 EF-hand domains.
MISCELLANEOUS: Binds two calcium ions; one with high affinity, the other with low affinity.
SIZE: 200 amino acids; 23130 Da
TISSUE SPECIFICITY: Retina and pineal gland.
DISEASE: SwissProt: P35243 # Identified as the antigen in cancer-associated retinopathy, an autoimmune disease of the retina caused by a tumor in another tissue.
SIMILARITY: SwissProt: P35243 ## Belongs to the recoverin family. & Contains 4 EF-hand domains.
MISCELLANEOUS: Binds two calcium ions; one with high affinity, the other with low affinity.
Qty/Pk:
100 µL
Brand Family:
Chemicon
Format:
Serum
Host:
Rabbit
Product Name:
Anti-Recoverin
Concentration:
Variable
Antibody Type:
Polyclonal Antibody