注文に関する情報
- : AB5585
- : 100 µL
- : Variable
画像情報
Rabbit anti-Recoverin (Catalog Number AB5585). Immunolocalization of recoverin in human retina.
Anti-Recoverin
当該製品は右記製品の推奨代替品です: AB5431P| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| H, M, R, Ch, Fg | IC, IH, IH(P), WB | Rabbit | Serum | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P35243 # Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
SIZE: 200 amino acids; 23130 Da
TISSUE SPECIFICITY: Retina and pineal gland.
DISEASE: SwissProt: P35243 # Identified as the antigen in cancer-associated retinopathy, an autoimmune ... もっと見る »
SIZE: 200 amino acids; 23130 Da
TISSUE SPECIFICITY: Retina and pineal gland.
DISEASE: SwissProt: P35243 # Identified as the antigen in cancer-associated retinopathy, an autoimmune ... もっと見る »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes a member of the recoverin family of neuronal calcium sensors. The encoded protein contains three calcium-binding EF-hand domains and may prolong the termination of the phototransduction cascade in the retina by blocking the phosphorylation of photo-activated rhodopsin. Recoverin may be the antigen responsible for cancer-associated retinopathy.
Description:
Anti-Recoverin
Replaces:
AB5431P
Background Information:
Recoverin is a calcium-binding protein expressed in retinal photoreceptors and midget cone bipolar cells. It appears to delay the termination of the phototransduction cascade by blocking the phosphorylation of photoexcited rhodopsin.
Alternate Names:
Recoverin
Specificity:
Recognizes recoverin.
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
- Western Blotting
Application Notes:
Western Blot Analysis:
1:1,000-1:5,000 dilution using ECL.
Immunohistochemistry:
1:1,000 (2 hours at room temperature or overnight at 2-8ºC) on frozen and paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde or 3% paraformaldehyde with 0.1% glutaraldehyde. Suggested permeabilization solution is 0.1% Triton X-100. Suggested blocking buffer is 0.05 M Tris saline, pH 7.4 containing 2.5% BSA, 0.1% triton and 0.05% sodium azide.
Optimal working dilutions must be determined by the end user.
1:1,000-1:5,000 dilution using ECL.
Immunohistochemistry:
1:1,000 (2 hours at room temperature or overnight at 2-8ºC) on frozen and paraffin embedded tissue sections. Suggested fixative is 4% paraformaldehyde or 3% paraformaldehyde with 0.1% glutaraldehyde. Suggested permeabilization solution is 0.1% Triton X-100. Suggested blocking buffer is 0.05 M Tris saline, pH 7.4 containing 2.5% BSA, 0.1% triton and 0.05% sodium azide.
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Human
- Mouse
- Rat
- Chicken
- Frog
Species Reactivity Note:
Human, rat, mouse, chicken and frog. Other species have not been tested.
Immunogen:
Recombinant human recoverin.
Molecular Weight:
26 kDa
Control:
POSITIVE CONTROL: Recoverin is found in photoreceptors and midget cone bipolar cells in retina and pinealocytes in pineal gland.
Presentation:
Rabbit polyclonal serum. Contains no preservative.
Storage Conditions:
Stable for 1 year at -20ºC from date of receipt.
Gene Symbol:
- RCVRN
- Recoverin
- RCV1
Antibody Category:
Neuroscience
Antibody Sub-Category:
Sensory & PNS
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Serum
Host:
Rabbit
Concentration:
Variable
Antibody Type:
Polyclonal Antibody