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Goat anti-Neuroketal (Catalog Number AB5611) Western blot of young normal brain homogenate.
Anti-Neuroketal
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | ELISA, WB, IH(P) | Goat | Serum | Polyclonal Antibody |
Description:
Anti-Neuroketal
Background Information:
Neuroketals and neuroprostanes are a class of compounds that result from the oxidation of docosahexenoic acid (DHA), which is enriched in the brain and retina, especially the synaptic membranes and retina. DHA is a membrane polyunsaturated fatty acid that is especially vulnerable to free radical attack because hydrogen radicals easily remove its double bonds. The DHA is oxidized to isoprostane-like compounds called neuroprostanes, which can dehydrate to form highly reactive A4/J4 neuroprostanes. Neuroprostanes can also undergo rearrangement to form D- and E-ring neuroprostanes. These reactive neuroprostanes are called neuroketals because DHA is so concentrated in the nervous system.
The fact that DHA is prone to free radical attack and free radicals has been implicated in a number of neurodegenerative diseases (Alzheimer's, Parkinson's, Huntington's, amyotrophic lateral sclerosis and AIDS dementia) may make neuroketals a unique and prominent marker of oxidative injury in the brain. The brain may especially susceptible to oxidative injury due to its high content of polyunsaturated fatty acids, its high oxygen consumption rate and it's relative lack of antioxidant defenses. NK protein adducts have been shown to occur in normal human brain, suggesting ongoing oxidative stress in the brain. Adduction of critical proteins by NK may be highly injurious to neurons and may be very important in the pathogenesis of neurodegenerative diseases associated with increased oxidative stress. Since neuroprostanes are increased in the brains and CSF of Alzheimer's disease patients, reactive NK formed through the neuroprostane pathway may play a major role in oxidative brain injury.
The fact that DHA is prone to free radical attack and free radicals has been implicated in a number of neurodegenerative diseases (Alzheimer's, Parkinson's, Huntington's, amyotrophic lateral sclerosis and AIDS dementia) may make neuroketals a unique and prominent marker of oxidative injury in the brain. The brain may especially susceptible to oxidative injury due to its high content of polyunsaturated fatty acids, its high oxygen consumption rate and it's relative lack of antioxidant defenses. NK protein adducts have been shown to occur in normal human brain, suggesting ongoing oxidative stress in the brain. Adduction of critical proteins by NK may be highly injurious to neurons and may be very important in the pathogenesis of neurodegenerative diseases associated with increased oxidative stress. Since neuroprostanes are increased in the brains and CSF of Alzheimer's disease patients, reactive NK formed through the neuroprostane pathway may play a major role in oxidative brain injury.
Specificity:
Neuroketal-adducted proteins. The antibody has been shown to react with neuroketal/neuroprostane-modified protein by ELISA. By Western blot the antibody reacts with several major and many minor bands in human brain protein modifications.
Key Applications:
- ELISA
- Western Blotting
- Immunohistochemistry (Paraffin)
Application Notes:
Western blot: >1:2,000
Immunohistochemistry: >1:200 on paraffin embedded tissue sections.
ELISA: >1:4,000
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: >1:200 on paraffin embedded tissue sections.
ELISA: >1:4,000
Optimal working dilutions must be determined by the end user.
Species Reactivity:
Human
Immunogen:
Neuroketal-conjugate.
Presentation:
Goat Serum. Liquid containing 0.01% thimerosal.
Storage Conditions:
Maintain at -20°C in undiluted for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Antibody Category:
Neuroscience
Antibody Sub-Category:
Neurodegenerative Diseases
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Serum
Host:
Goat
Antibody Type:
Polyclonal Antibody