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Ordering Information
- : AB5971
- : 100 µg
Anti-HuD
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IH | Rabbit | Affinity Purified | Polyclonal Antibody |
Description:
Anti-HuD
Trade Name:
Chemicon (Millipore)
Specificity:
Recognizes HuD. The molecular weight of the protein is 41,766 Daltons.
Immunogen:
Synthetic peptide, amino acids 26-42 of HuD.
Species Reactivity:
- Human
- Mouse
Application Notes:
Western blot: 1:200-1:1,000 using ECL.
Immunohistochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.
Presentation:
Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Storage Conditions:
Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
PREPARATION AND USE:
To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.
Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.
Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.
The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.
For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
PREPARATION AND USE:
To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.
Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.
Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.
The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.
For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- ELAVL4
- PNEM
- HUD
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Western Blotting
- Immunohistochemistry
UniProt Summary:
FUNCTION: SwissProt: P26378 # May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity).
SIZE: 380 amino acids; 41760 Da
SUBUNIT: Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP (By similarity).
TISSUE SPECIFICITY: Brain.
PTM: Methylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth (By similarity).
DISEASE: SwissProt: P26378 # Paraneoplastic encephalomyelitis sensory neuropathy is associated with small cell lung cancer and is characterized by dementia, sensory loss, and other neurological disabilities. It may result from an immune response primarily directed against a small cell lung tumor antigen which is misdirected against similar antigens expressed in brain.
SIMILARITY: SwissProt: P26378 ## Belongs to the RRM elav family. & Contains 3 RRM (RNA recognition motif) domains.
SIZE: 380 amino acids; 41760 Da
SUBUNIT: Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP (By similarity).
TISSUE SPECIFICITY: Brain.
PTM: Methylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth (By similarity).
DISEASE: SwissProt: P26378 # Paraneoplastic encephalomyelitis sensory neuropathy is associated with small cell lung cancer and is characterized by dementia, sensory loss, and other neurological disabilities. It may result from an immune response primarily directed against a small cell lung tumor antigen which is misdirected against similar antigens expressed in brain.
SIMILARITY: SwissProt: P26378 ## Belongs to the RRM elav family. & Contains 3 RRM (RNA recognition motif) domains.
Qty/Pk:
100 µg
Brand Family:
Chemicon
Format:
Affinity Purified
Host:
Rabbit
Product Name:
Anti-HuD
Antibody Type:
Polyclonal Antibody