Goat anti-Mouse IgG, Peroxidase Conjugated, H+L
| 交差性 | 主要アプリケーション | 免疫動物 | フォーマット | 抗体タイプ |
|---|---|---|---|---|
| M | ELISA, IH, WB | Goat | HRP | Polyclonal Antibody |
Description:
Goat anti-Mouse IgG, Peroxidase Conjugated, H+L
Background Information:
Affinity purified goat anti-mouse IgG, Peroxidase conjugated.
Specificity:
Reacts with mouse IgG under native and reduced conditons. Under reduced conditions cross reactivity to non-mouse IgG may be seen because of similarity of immunoglobulin chains.
Key Applications:
- ELISA
- Immunohistochemistry
- Western Blotting
Application Notes:
ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
1:5,000-1:100,000 dilution can be used.
Immunohistochemistry:
1:500-1:5,000 dilution can be used.
Optimal working dilutions must be determined by end user.
Species Reactivity:
Mouse
Isotype:
IgG
Presentation:
Purified goat IgG conjugated to horseradish peroxidase in buffer containing 0.01 M Phosphate Buffered Saline, pH 7.1 with 15 mg/mL BSA and 0.01% Thimerosal. Lyophilized.
Storage Conditions:
Maintain lyophilized product at 2-8°C for up to 12 months from date of shipment.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.
Antibody Category:
Secondary & Control Antibodies
Antibody Sub-Category:
Whole Immunoglobulin Secondary Antibodies
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
HRP
Host:
Goat
Product Overview:
Affinity purified goat anti-mouse IgG, Peroxidase conjugated.
Reagent Type:
Western Blotting Reagents
Format Code:
HRP
Concentration:
1 mg/mL (after reconstitution)
Antibody Type:
Polyclonal Antibody
Purification Method:
Immunoaffinity Purified


