DNA Ladder Detection Kit

DNA Ladder Detection Kit

Chemicon (Millipore)

Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. Chemicon's Quick Apoptotic DNA Ladder Detection Kit provides a fast and easy means for detection of DNA fragmentation in apoptotic cells. Unlike other similar kits which require 1-2 days to finish the procedure, the new detection method requires less than 90 minutes to prepare DNA in a single tube, without the need for extraction or the use of columns. DNA fragmentation can be easily visualized by agarose gel electrophoreses. The new procedure increases recovery of small fragmented DNA and therefore improves the sensitivity of the assay.

Activity Assay

Mammals

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

• Apoptosis Assays
• Apoptosis Assays

• TE Lysis Buffer - 2 mL.
• Enzyme A Solution - 0.25 mL.
• Enzyme B* - 1 vial
• Ammonium Acetate Solution - 0.25 mL.
• DNA Suspension Buffer - 2 mL.
• Note: Dissolve Enzyme B with 275 μL TE Lysis Buffer and mix well before use. The unused Enzyme B solution should be frozen at -70°C.

We recommend storing reagents at -20°C upon arrival. Frequent freeze-thawing is not recommended. The performance of this product is guaranteed for 6 months.

Chromogenic

· Microcentrifuge and 1.5 mL microcentrifuge tubes

· 55°C water bath or incubator

· Phosphate buffered Saline solution (PBS) - [10 mM Na2PO4; 138 mM NaCl; 2.7 mM KCl]

· Absolute ethanol

· Equipment and reagents for agarose gel electrophoresis and DNA visualization