Ordering Information
Anti-VCAM-1, clone M/K - 2
Recommended Replacement for: MAB2242| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IH, IP, INHIB | Rat | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P19320 # Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
SIZE: 739 amino acids; 81276 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protei ... see more »
SIZE: 739 amino acids; 81276 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protei ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene.
Description:
Anti-VCAM-1, clone M/K - 2
Replaces:
MAB2242
Alternate Names:
CD106
Clone:
M/K - 2
Specificity:
Recognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.
Key Applications:
- Flow Cytometry
- Immunohistochemistry
- Immunoprecipitation
- Inhibits Activity/Function
Application Notes:
Studies of cell-cell interaction
Cell adhesion assays
Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures
Immunoprecipitation of the VCAM-1 integrin complex
Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.
Optimal working dilutions must be determined by the end user.
Cell adhesion assays
Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures
Immunoprecipitation of the VCAM-1 integrin complex
Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.
Optimal working dilutions must be determined by the end user.
Species Reactivity:
Mouse
Isotype:
IgG1κ
Control:
EOMA and BMS2 endothelial cell lysates
Presentation:
The monoclonal is presented in phosphate buffered saline containing 10mM sodium azide.
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Gene Symbol:
- VCAM1
- INCAM-100
- MGC99561
- CD106
- DKFZp779G2333
- L1CAM
Antibody Category:
Cell Structure
Antibody Sub-Category:
Adhesion (CAMs)
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Purified
Host:
Rat
Format Code:
Pur
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Purification Method:
Protein A Purified