Ordering Information
- : CBL1337
- : 500 μg
- : 0.5 mg/mL
Anti-PECAM-1, clone 390
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | FC, IH, IP, FUNC | Rat | Purified | Monoclonal Antibody |
Description:
Anti-PECAM-1, clone 390
Trade Name:
Chemicon (Millipore)
Specificity:
CBL1337 recognizes mouse PECAM-1, a 130-140 kDa type 1 integral membrane glycoprotein. This antibody has been shown to inhibit the aggregation of L cells transfected with a variant form of PECAM-1 (DeLisser, 1993).
Immunogen:
Mouse leukocyte cell line 32D
Clone:
390
Isotype:
IgG2aκ
Background Information:
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1) is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.
Species Reactivity:
Mouse
Application Notes:
Flow Cytometry: 1 μg/10e6 cells.
Immunohistochemistry: acteone fixed frozen tissue sections
Immunoprecipitation
Blocking of cell adhesion: CBL1337 inhibits aggregation of L cells transfected with a variant form of CD31 (DeLisser, 1993)
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: acteone fixed frozen tissue sections
Immunoprecipitation
Blocking of cell adhesion: CBL1337 inhibits aggregation of L cells transfected with a variant form of CD31 (DeLisser, 1993)
Optimal working dilutions must be determined by the end user.
Control:
Jurkat and Raji cells, tonsil tissue
Purification Method:
Protein A purfied
Presentation:
Protein A Purified mouse immunoglobulin in 100mM borate buffered saline, pH 8.0, containing no preservative.
Storage Conditions:
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
UniProt Number:
Entrez Gene Number:
Gene Symbol:
- PECAM1
- EndoCAM
- GPIIA'
- PECAM-1
- CD31
- CD31/EndoCAM
Alternate Names:
CD31
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications:
- Flow Cytometry
- Immunohistochemistry
- Immunoprecipitation
- Affects Function
UniProt Summary:
FUNCTION: SwissProt: P16284 # This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.
SIZE: 738 amino acids; 82536 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.
PTM: Phosphorylated on Ser and Tyr residues after cellular activation.
SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
SIZE: 738 amino acids; 82536 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.
PTM: Phosphorylated on Ser and Tyr residues after cellular activation.
SIMILARITY: SwissProt: P16284 ## Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Brand Family:
Chemicon
Product Name:
Anti-PECAM-1, clone 390
Concentration:
0.5 mg/mL
Antibody Type:
Monoclonal Antibody
Qty/Pk:
500 μg
Format:
Purified
Host:
Rat