Canine C-Peptide RIA
Description:
Canine C-Peptide RIA
Background Information:
C-peptide is a byproduct of normal insulin production by the beta cells in the pancreas and correlates directly to endogenous concentrations of insulin.
Key Applications:
Radioimmunoassay
Application Notes:
Overnight equilibrium incubation at 4°C. Sample volume < 100 µL serum, plasma, or tissue culture media. Dilution: Antibody 1:32,000 titer
Species Reactivity:
Canine
Precision, %:
- Inter-assay: 5.61-6.91
- Intra-assay: 3.06-3.33
Therapeutic Areas:
- Diabetes
- Obesity
- Metabolic Disorders
Sensitivity:
0.15 ng/mL
Accuracy:
97-111%
Standard Curve Range:
0.156–20 ng/mL
Label:
125I-Canine C-Peptide
Antigen:
aa1-aa31-OVA via glutaraldehyde
Kit Type:
Endocrine Kits
Detection Methods:
125I
Linearity of Dilution:
95-101%
Kit or Assay Type:
- Endocrine Kits
- Radioimmunoassays (RIA)
Radioactivity:
<3 µCi
Performance:
- ED80 = 0.56 ± 0.15 ng/mL
- ED50 = 2.03 ± 0.54 ng/mL
- ED20 = 7.21 ± 2.76 ng/mL
Assay Type:
Radioimmunoassays (RIA)