Airway Epithelium Medium (Human)
Description:
Airway Epithelium Medium (Human)
Product Overview:
CnT-23 is a liquid culture medium kit including both basal medium and separate supplements, developed for maximum efficiency differentiation of airway epithelium. CnT-23 is fully defined, low calcium, serum and BPE-free formulation, which contains 10nM retinoic acid. It does not contain antibiotics or antimycotics. The basal medium contains amino acids, minerals, vitamins and organic compounds, but is protein free.
Key Applications:
Cell Culture
Application Notes:
Developed for differentiation of airway epithelia. For suggested seeding densities, please refer to "Suggested Seeding Densities for CELLnTEC Media" in protocols tab. Formulated for human epithelium, but may be used for other species as well. If using CnT-17 to isolate airway epithelium, cells should be transferred from CnT-17 medium to CnT-23 medium prior to induction of differentiation. This medium requires the addition of calcium to trigger differentiation. See Differentiation Protocol for further details.
Antibiotics/ Antimycotics may be added if desired; CELLnTEC products CnT-ABM10, CnT-ABM20 or CnT-ABM will provide a final concentration of 100 U/mL Penicillin, 100 µg/mL Streptomycin and 250 ng/mL Amphotericin B.
This medium is designed for use in a 5% CO2 atmosphere.
For suggested cell culture protocols, please see www.cellntec.com.
Antibiotics/ Antimycotics may be added if desired; CELLnTEC products CnT-ABM10, CnT-ABM20 or CnT-ABM will provide a final concentration of 100 U/mL Penicillin, 100 µg/mL Streptomycin and 250 ng/mL Amphotericin B.
This medium is designed for use in a 5% CO2 atmosphere.
For suggested cell culture protocols, please see www.cellntec.com.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Reagent Type:
Cell Culture Media
Media Form:
Liquid
Media Type:
Cell Culture Media
Components:
- Basal Media (CnT-23.BM), 500mls
- Supplement (CnT-23.S); Containing three Supplements (A, B and C), frozen (To be added to 500 mL basal medium prior to use)
Quality Assurance:
Certified as free of bacteria, fungi, and mycoplasma.
Sales Restrictions:
This product is not available for sale in Japan.
Storage Conditions:
Basal Media (CnT-23.BM): Store at 4°C in the dark. See label for expiration date.
Supplement (CnT-23.S): Store at -20°C. See label for expiration date.
Supplemented medium without retinoic acid: Store at 4°C in the dark. Use within 3 months of supplementation. Do not freeze. Supplemented medium with retinoic acid: Store at 4°C, completely protected from light and with minimized oxygen exposure (store in full containers). Use within 1 month of supplementation. Do not freeze.
Supplement (CnT-23.S): Store at -20°C. See label for expiration date.
Supplemented medium without retinoic acid: Store at 4°C in the dark. Use within 3 months of supplementation. Do not freeze. Supplemented medium with retinoic acid: Store at 4°C, completely protected from light and with minimized oxygen exposure (store in full containers). Use within 1 month of supplementation. Do not freeze.