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PrecisION hNav1.6-HEK Recombinant Cell Line
Description:
PrecisION hNav1.6-HEK Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant HEK293 cell line expressing the human Nav1.6 (type VIII voltage-gated sodium channel alpha subunit).
Format:
2 x 1 ml aliquots containing 12.22 x 106cells/ml in 10% DMSO at passage 11.
Format:
2 x 1 ml aliquots containing 12.22 x 106cells/ml in 10% DMSO at passage 11.
Background Information:
hNav1.6 is primarily expressed in the output neurones of the cerebellum, cortex and hippocampus of the CNS and in the Nodes of Ranvier of sensory and motor axons of the PNS. hNav1.6 is involved with the initiation and transmission of action potentials in central neurons and their myelinated axons. Mutation or disruption of the Nav1.6 gene causes cerebellar ataxia and motor end plate disease in mice. This channel is a potential target for analgesic and antiepileptic drugs.
Application Notes:
HEK293 cells stably expressing hNav1.6 were analyzed by patch-clamp and IonWorks™. Typically, >90% of cells sealed with >80% of cells expressing giving mean peak currents of 1.42 nA (n=313). IC50 values obtained for the known hNav1.6 inhibitors were 2.5 nM for tetrodotoxin and for lamotrigine (Lamictal) were 486 μM, 127 μM, and 31 μM at VH of -120 mV, -90 mV & -60 mV respectively.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
FUNCTION: SwissProt: Q9UQD0 # Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.
SIZE: 1980 amino acids; 225280 Da
SUBUNIT: Interacts with NEDD4 and NEDD4L (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: Q9UQD0 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
PTM: May be ubiquitinated by NEDD4L; which would promote its endocytosis (By similarity).
SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
SIZE: 1980 amino acids; 225280 Da
SUBUNIT: Interacts with NEDD4 and NEDD4L (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: Q9UQD0 The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.
PTM: May be ubiquitinated by NEDD4L; which would promote its endocytosis (By similarity).
SIMILARITY: Belongs to the sodium channel family. & Contains 1 IQ domain.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
HEK
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
293 cell line
Presentation:
2 x 1 ml aliquots
Protein Target:
hNav1.6
UniProt Number:
Target Sub-Family:
Sodium
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- SCN8A
- MED
- NaCh6
- PN4
- Nav1.6
- CerIII
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hNav1.6-HEK Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- SCN8A
- MED
- NaCh6
- PN4
- Nav1.6
- CerIII