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PrecisION hHCN4-CHO Recombinant Cell Line
Description:
PrecisION hHCN4-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing the human HCN4 (hyperpolarization activated cyclic nucleotide-gated potassium channel 4).
Format:
2 x 1 ml aliquots containing 1.25 x106cells/ml in 10% DMSO at passage 14.
Format:
2 x 1 ml aliquots containing 1.25 x106cells/ml in 10% DMSO at passage 14.
Background Information:
HCN4 is the major Hyperpolarization-activated Cyclic Nucleotide-gated ion channel in the mammalian heart. They are voltage-gated, cation channels, typically closed at resting and depolarized membrane potentials. The action of HCN4 is essential for the rhythmicity of the heart beat as well as controlling the strength of the contraction. HCN4 is expressed in the heart and brain, but significantly, not in the retina. The side effects of many anti-arrhythmic drugs include visual disturbances; a selective HCN4 blocker would potentially slow the heart rate without affecting vision. Safety screening, to detect undesirable action on HCN4 channels, is essential to assess the cardiac liability of new drug submissions.
Application Notes:
CHO-K1 cells stably expressing hHCN4 were analyzed by IonWorks™ HT. Over 88% of cells expressed >300 pA outward current (n=1099) with a mean current amplitude of 1360 pA ± 0.03 (n=971). The HCN4 current was inhibited by standard blockers such as capsazepine (pIC50 = 4.2 ± 0.1; n=3) and ZD-7288 (pIC50 = 4.4 ± 0.2; n=4).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UniProt Summary:
FUNCTION: SwissProt: Q9Y3Q4 # Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli.
SIZE: 1203 amino acids; 129042 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. Detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus.
DOMAIN:SwissProt: Q9Y3Q4 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
DISEASE: SwissProt: Q9Y3Q4 # Defects in HCN4 are a cause of autosomal dominant sick sinus syndrome (SSS) [MIM:163800]; also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors, in which case it is considered to be a congenital disorder.
SIMILARITY: Belongs to the potassium channel HCN family. & Contains 1 cyclic nucleotide-binding domain.
MISCELLANEOUS: Inhibited by extracellular cesium ions.
SIZE: 1203 amino acids; 129042 Da
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. Detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus.
DOMAIN:SwissProt: Q9Y3Q4 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
DISEASE: SwissProt: Q9Y3Q4 # Defects in HCN4 are a cause of autosomal dominant sick sinus syndrome (SSS) [MIM:163800]; also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. The term 'sick sinus syndrome' encompasses a variety of conditions caused by sinus node dysfunction. The most common clinical manifestations are syncope, presyncope, dizziness, and fatigue. Electrocardiogram typically shows sinus bradycardia, sinus arrest, and/or sinoatrial block. Episodes of atrial tachycardias coexisting with sinus bradycardia ('tachycardia-bradycardia syndrome') are also common in this disorder. SSS occurs most often in the elderly associated with underlying heart disease or previous cardiac surgery, but can also occur in the fetus, infant, or child without heart disease or other contributing factors, in which case it is considered to be a congenital disorder.
SIMILARITY: Belongs to the potassium channel HCN family. & Contains 1 cyclic nucleotide-binding domain.
MISCELLANEOUS: Inhibited by extracellular cesium ions.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Presentation:
2 x 1 ml aliquots
Protein Target:
hHCN4
UniProt Number:
Target Sub-Family:
Hyperpolarized
Packaging:
2 x 1 ml aliquots
Gene Symbol:
HCN4
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hHCN4-CHO Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
HCN4