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PrecisION hKv1.1-CHO Recombinant Cell Line
Description:
PrecisION hKv1.1-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing human Kv1.1.
Format:
2 x 1 ml aliquots containing 1.60 x 106cells/ml in 10% DMSO at passage 8.
Format:
2 x 1 ml aliquots containing 1.60 x 106cells/ml in 10% DMSO at passage 8.
Background Information:
Kv1.1 is a voltage-gated, delayed rectifier, potassium channel. It is expressed in the brain, heart, retina, skeletal muscle and pancreatic islets and is primarily involved with maintaining the resting membrane potential and modulating electrical excitability in neurons and muscle cells. Mutations of this ion channel have been linked to episodic ataxia, an autosomal dominant human disorder that produces persistent myokymia (successive, involuntary, small muscle contractions or tics that affect a bundle of muscles).
Application Notes:
CHO-K1 cells expressing hKv1.1 were characterized in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques. The mean current amplitude at 60 mV was 860 ± 30 pA. The threshold of activation was around -40 mV and exhibited slow C-type inactivation at depolarized potentials. These properties are characteristic of Kv1.1 currents described in the literature. The cell-line was shown to be selectively expressing Kv1.1 current since it was completely blocked by low nM concentrations of the toxin blocker, Dendrotoxin K and low mM concentrations of TEA. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression increased over at least 33 passages: 44% of cells expressed Kv1.1 currents at passage 33 giving a mean current amplitude of 860 ± 30 pA at 60 mV (n=64).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
This gene encodes a voltage-gated delayed potassium channel that is phylogenetically related to the Drosophila Shaker channel. The encoded protein has six putative transmembrane segments (S1-S6), and the loop between S5 and S6 forms the pore and contains the conserved selectivity filter motif (GYGD). The functional channel is a homotetramer. The N-terminus of the channel is associated with beta subunits that can modify the inactivation properties of the channel as well as affect expression levels. The C-terminus of the channel is complexed to a PDZ domain protein that is responsible for channel targeting. Mutations in this gene have been associated with myokymia with periodic ataxia (AEMK).
UniProt Summary:
FUNCTION: SwissProt: Q09470 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
SIZE: 495 amino acids; 56542 Da
SUBUNIT: Heterotetramer of potassium channel proteins. Binds KCNAB2 and PDZ domains of DLG1, DLG2 and DLG4 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: Q09470 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
PTM: Palmitoylated on Cys-243; which may be required for membrane targeting.
DISEASE: SwissProt: Q09470 # Defects in KCNA1 are the cause of myokymia with periodic ataxia (AEMK) [MIM:160120]; also known as paroxysmal or episodic ataxia with myokymia (EAM) or episodic ataxia type 1 (EA-1). AEMK is an autosomal dominant disorder characterized by brief episodes of ataxia and dysarthria. Neurological examination during and between the attacks demonstrates spontaneous, repetitive discharges in the distal musculature (myokymia) that arise from peripheral nerve. Nystagmus is absent.
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.
SIZE: 495 amino acids; 56542 Da
SUBUNIT: Heterotetramer of potassium channel proteins. Binds KCNAB2 and PDZ domains of DLG1, DLG2 and DLG4 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: Q09470 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
PTM: Palmitoylated on Cys-243; which may be required for membrane targeting.
DISEASE: SwissProt: Q09470 # Defects in KCNA1 are the cause of myokymia with periodic ataxia (AEMK) [MIM:160120]; also known as paroxysmal or episodic ataxia with myokymia (EAM) or episodic ataxia type 1 (EA-1). AEMK is an autosomal dominant disorder characterized by brief episodes of ataxia and dysarthria. Neurological examination during and between the attacks demonstrates spontaneous, repetitive discharges in the distal musculature (myokymia) that arise from peripheral nerve. Nystagmus is absent.
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Presentation:
2 x 1 ml aliquots
Protein Target:
hKv1.1
UniProt Number:
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- KCNA1
- HUK1
- Kv1.1
- HUKI
- RBK1
- MGC126782
- HBK1
- AEMK
- EA1
- MBK1
- KV1.1
- MK1
- MGC138385
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hKv1.1-CHO Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- KCNA1
- HUK1
- Kv1.1
- HUKI
- RBK1
- MGC126782
- HBK1
- AEMK
- EA1
- MBK1
- KV1.1
- MK1
- MGC138385