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PrecisION hKv1.2-CHO Recombinant Cell Line
Description:
PrecisION hKv1.2-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing human Kv1.2.
Format:
2 x 1 ml aliquots containing 1.204 x 106cells/ml in 10% DMSO at passage 9.
Format:
2 x 1 ml aliquots containing 1.204 x 106cells/ml in 10% DMSO at passage 9.
Background Information:
Kv1.2 frequently forms heteromultimers with other Kv1.x subunits contributing to the delayed rectifier potassium current in a variety of tissues. The channel is expressed throughout the brain, spinal cord, Schwann cells , the heart atria and ventricles, pancreatic islets, retina and smooth muscle. It is primarily involved with maintaining the resting potential of cells and modulating electrical excitability in neurons and muscles
Application Notes:
CHO-K1 cells expressing hKv1.2 were characterized in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques. The mean current amplitude at 40 mV was 2000 &plusms; 570 pA (n=6). The threshold of current activation was around -10 mV and exhibited slow C-type inactivation at voltages >/= 40 mV. The current activates very slowly between -10 mV and 20 mV. These electrophysiological properties are consistent with previous reports in the literature describing Kv1.2 currents. The cell-line was shown to be selectively expressing hKv1.2 current since it was blocked by low nM concentrations of the toxin blocker, Tityustoxin Kα and resistant to low mM concentrations of TEA. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 32 passages: 91% of cells expressed Kv1.2 currents (>500 pA) at passage 32 giving a mean current amplitude of 1390 ± 69 pA at 60 mV (n=114).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1.
UniProt Summary:
FUNCTION: SwissProt: P16389 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
SIZE: 499 amino acids; 56717 Da
SUBUNIT: Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: P16389 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.
SIZE: 499 amino acids; 56717 Da
SUBUNIT: Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: P16389 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Presentation:
2 x 1 ml aliquots
Protein Target:
hKv1.2
UniProt Number:
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- KCNA2
- HBK5
- MGC50217
- RBK2
- HK4
- MK2
- KV1.2
- NGK1
- HUKIV
- Kv1.2
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hKv1.2-CHO Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- KCNA2
- HBK5
- MGC50217
- RBK2
- HK4
- MK2
- KV1.2
- NGK1
- HUKIV
- Kv1.2