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PrecisION hKv1.4-CHO Recombinant Cell Line
Description:
PrecisION hKv1.4-CHO Recombinant Cell Line
Trade Name:
Upstate (Millipore)
Qty/Pk:
2 mL
Product Overview:
Recombinant CHO-K1 cell line expressing human Kv1.4.
Format:
2 x 1 ml aliquots containing 2.68x 106cells/ml in 10% DMSO at passage 11.
Format:
2 x 1 ml aliquots containing 2.68x 106cells/ml in 10% DMSO at passage 11.
Background Information:
Kv1.4 is a voltage-gated, fast-inactivating, A-type potassium ion channel also known as HuK(II), hPCN2, HK1, RCK4, RHK1, RK4, RK8 and MK4. It is expressed in the CNS, lung, skeletal muscle, heart and pancreatic islet cells. It is responsibly for neuronal afterhypolarization. The expression of Kv1.4 has been shown to increase after induction of diabetes and in ventricular myocytes after a heart attack
Application Notes:
CHO-K1 cells expressing hKv1.4 were characterised in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques. The mean current amplitude at 0 mV was 3600 ± 2700 pA (n=3). The threshold of activation was around -40 mV and exhibited fast N-type inactivation. The kinetics of inactivation and the current/voltage relationship are consistent with published data describing the Kv1.4 current. The current was blocked by 1 mM 4-AP to an extent expected for selective expression of Kv1.4 channels. The percent inhibition of the current using 1 mM 4-AP was 71 ± 6% (n=4). The current was also inhibited by mild acidosis in agreement with previous reports describing the Kv1.4 current. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 36 passages: 95% of cells expressed Kv1.4 currents at passage 35 giving a mean current amplitude of 4.64 ± 0.22 nA at 60 mV (n=141).
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Entrez Gene Summary:
Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the A-type potassium current class, the members of which may be important in the regulation of the fast repolarizing phase of action potentials in heart and thus may influnce the duration of cardiac action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1.
UniProt Summary:
FUNCTION: SwissProt: P22459 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
SIZE: 653 amino acids; 73288 Da
SUBUNIT: Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4. Interacts with OPRS1 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: P22459 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.
SIZE: 653 amino acids; 73288 Da
SUBUNIT: Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4. Interacts with OPRS1 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: P22459 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.
Species:
Human
Quality Assurance:
Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species
The cell line has been screened to confirm the absence of Mycoplasma species
Cell Type:
CHO
Cell Line Type:
Ion Channel Cell Lines
Components:
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial
Brand Family:
Upstate
Host Cells:
Chinese Hamster Ovarian K-1 cells (CHO K-1)
Presentation:
2 x 1 ml aliquots
Protein Target:
hKv1.4
UniProt Number:
Target Sub-Family:
Potassium
Packaging:
2 x 1 ml aliquots
Gene Symbol:
- KCNA4
- HPCN2
- KV1.4
- HBK4
- PCN2
- HUKII
- Kv1.4
- KCNA4L
- KCNA8
- HK1
Protein or Enzyme Type:
Ion Channels
Product Name:
PrecisION hKv1.4-CHO Recombinant Cell Line
Entrez Gene Number:
Alternate Names:
- KCNA4
- HPCN2
- KV1.4
- HBK4
- PCN2
- HUKII
- Kv1.4
- KCNA4L
- KCNA8
- HK1