PrecisION hKv1.5-CHO Recombinant Cell Line

PrecisION hKv1.5-CHO Recombinant Cell Line

Upstate (Millipore)

2 mL

Recombinant CHO-K1 cell line expressing human Kv1.5.

Format:
2 x 1 ml aliquots containing 2.08 x 10⁶cells/ml in 7.5% DMSO at passage 10.

Kv1.5 is expressed in the brain (hippocampus, pituitary, microglia, oligodendrocytes and Schwann cells) as well as kidney, colon, aorta, pulmonary artery and smooth muscle. This ion channel is involved in the maintenance of the resting membrane potential and therefore regulates the activity electrically excitable cells. Kv1.5 is potentially a target for the management of atrial fibrillation. Since they are not found in the ventricles, drugs selective for this channel may be beneficial in the treatment of atrial fibrillation without the risk of causing ventricular arrhythmias. Apart from these instances where the purpose is to develop a cardiovascular drug targeted to Kv1.5, an interaction of lead development compounds with this channel is best avoided. This is due to the fact that the channel has such a widespread distribution and controls membrane excitability

CHO-K1 cells expressing hKv1.5 were characterized in terms of their pharmacological and biophysical properties using whole-cell patch clamp techniques. The mean current amplitude at 40 mV was 2340 ± 1000 pA (n=4). The threshold for current activation was around -30 mV and exhibited slow C-type inactivation at depolarized potentials. These properties are characteristic of Kv1.5 currents described in the literature. 4-Aminopyridine blocked the current in a dose-dependent manner (IC50 value =100 μM) within the concentration range reported for block of Kv1.5 channels (range 50-200 μM). Loratadine blocked the channel via the same mechanism described previously for Kv1.5 channels expressed in HEK293 cells. Functional channel expression over time was monitored using IonWorks™ HT. Channel expression is robust over at least 44 passages: 93% of cells expressed Kv1.5 currents of >500 pA at passage 44.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Potassium channels represent the most complex class of voltage-gated ino channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, the function of which could restore the resting membrane potential of beta cells after depolarization and thereby contribute to the regulation of insulin secretion. This gene is intronless, and the gene is clustered with genes KCNA1 and KCNA6 on chromosome 12.

FUNCTION: SwissProt: P22460 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potasssium ions may pass in accordance with their electrochemical gradient. May play a role in regulating the secretion of insulin in normal pancreatic islets.
SIZE: 613 amino acids; 67228 Da
SUBUNIT: Heterotetramer of potassium channel proteins. Interacts via its PDZ-binding motif with DLG1. Forms a ternary complex with DLG1 and CAV3 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Pancreatic islets and insulinoma.
DOMAIN: SwissProt: P22460 The amino terminus may be important in determining the rate of inactivation of the channel while the C-terminal PDZ- binding motif may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments (By similarity). & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.

Human

Mycoplasma Testing:
The cell line has been screened to confirm the absence of Mycoplasma species

CHO

Ion Channel Cell Lines

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial

Upstate

Chinese Hamster Ovarian K-1 cells (CHO K-1)

2 x 1 ml aliquots

hKv1.5

P22460

Potassium

2 x 1 ml aliquots

• KCNA5
• HPCN1
• KV1.5
• HK2
• HCK1
• PCN1
• MGC117059
• MGC117058
• Kv1.5

Ion Channels

PrecisION hKv1.5-CHO Recombinant Cell Line

NM_002234.2

• KCNA5
• HPCN1
• KV1.5
• HK2
• HCK1
• PCN1
• MGC117059
• MGC117058
• Kv1.5